Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase
defines an essential function for the Sgs1-Top3 complex in the
absence of SRS2 or TOP1.

J 2000

Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.

DUNO, Morten, Bo THOMSEN, Ole WESTERGAARD, Lumír KREJČÍ, Christian BENDIXEN et. al.

Basic information

Original name

Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.

Name in Czech

Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.

Authors

DUNO, Morten (208 Denmark), Bo THOMSEN (208 Denmark), Ole WESTERGAARD (208 Denmark), Lumír KREJČÍ (203 Czech Republic, guarantor) and Christian BENDIXEN (208 Denmark)

Edition

Mol Gen Genet, 2000, 1617-4615

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Denmark

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

Recombination; repair; Sgs1;Top3

Abstract

ORIG CZ

V originále

The Saccharomyces cerevisiae gene SGS1 encodes a DNA helicase that shows homology to the Escherichia coli protein RecQ and the products of the BLM and WRN genes in humans, which are defective in Bloom's and Werner's syndrome, respectively. Recently, it has been proposed that this helicase is involved in maintaining the integrity of the rDNA and that loss of Sgs1 function leads to accelerated aging. Sgs1 has been isolated on the basis of its genetic interaction with both topoisomerase I and topoisomerase III, as well as in a two-hybrid screen for proteins that interact with the C-terminal portion of topoisomerase II. We have defined the minimal structural elements of Sgs1 required for its interactions with the three topoisomerases, and demonstrate that the complex phenotypes associated with sgs1 mutants are a consequence of a dysfunctional Sgs1-Top3 complex. We also report that the synthetic relationship between mutations in SGS1 and SRS2, which encodes another helicase implicated in recombinational repair, likewise result from a dysfunctional Sgs1-Top3 interaction. Our findings indicate that Sgs1 may act on different DNA structures depending on the activity of topoisomerase I, Srs2 and topoisomerase III.

In Czech

Genetická charakterizace SGS1 a TOP1 vztahů

Citovat

DUNO, Morten, Bo THOMSEN, Ole WESTERGAARD, Lumír KREJČÍ and Christian BENDIXEN. Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1. Mol Gen Genet. 2000, vol. 264, 1-2, p. 89-97, 8 pp. ISSN 1617-4615.

@article{708570,
   author = {Duno, Morten and Thomsen, Bo and Westergaard, Ole and Krejčí, Lumír and Bendixen, Christian},
   article_number = {1-2},
   keywords = {Recombination; repair; Sgs1;Top3},
   language = {eng},
   issn = {1617-4615},
   journal = {Mol Gen Genet},
   title = {Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.},
   volume = {264},
   year = {2000}
}

TY  - JOUR
ID  - 708570
AU  - Duno, Morten - Thomsen, Bo - Westergaard, Ole - Krejčí, Lumír - Bendixen, Christian
PY  - 2000
TI  - Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1.
JF  - Mol Gen Genet
VL  - 264
IS  - 1-2
SP  - 89-97
EP  - 89-97
SN  - 16174615
KW  - Recombination
KW  - repair
KW  - Sgs1;Top3
N2  - The Saccharomyces cerevisiae gene SGS1 encodes a DNA helicase that shows homology to the Escherichia coli protein RecQ and the products of the BLM and WRN genes in humans, which are defective in Bloom's and Werner's syndrome, respectively. Recently, it has been proposed that this helicase is involved in maintaining the integrity of the rDNA and that loss of Sgs1 function leads to accelerated aging. Sgs1 has been isolated on the basis of its genetic interaction with both topoisomerase I and topoisomerase III, as well as in a two-hybrid screen for proteins that interact with the C-terminal portion of topoisomerase II. We have defined the minimal structural elements of Sgs1 required for its interactions with the three topoisomerases, and demonstrate that the complex phenotypes associated with sgs1 mutants are a consequence of a dysfunctional Sgs1-Top3 complex. We also report that the synthetic relationship between mutations in SGS1 and SRS2, which encodes another helicase implicated in recombinational repair, likewise result from a dysfunctional Sgs1-Top3 interaction. Our findings indicate that Sgs1 may act on different DNA structures depending on the activity of topoisomerase I, Srs2 and topoisomerase III.
ER  -

DUNO, Morten, Bo THOMSEN, Ole WESTERGAARD, Lumír KREJČÍ and Christian BENDIXEN. Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1. \textit{Mol Gen Genet}. 2000, vol.~264, 1-2, p.~89-97, 8 pp. ISSN~1617-4615.

Detailed Information on Publication Record