BUDILOVÁ, Jana, Jiří PAZOUREK, Pavel KRÁSENSKÝ a Jan POSPÍCHAL. A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., roč. 29, č. 11, s. 1613-1621. ISSN 1615-9306. 2006.
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Základní údaje
Originální název A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS
Název česky A CONTINUOUS MODE OF OPERATION FOR LARGE VOLUME DOSING IN ANALYTICAL CARRIER AMPHOLYTE–FREE ISOELECTRIC FOCUSING OF PROTEINS APPLIED TO OFF–LINE DETECTION OF FRACTIONS
Autoři BUDILOVÁ, Jana (203 Česká republika), Jiří PAZOUREK (203 Česká republika, garant), Pavel KRÁSENSKÝ (203 Česká republika) a Jan POSPÍCHAL (203 Česká republika).
Vydání Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2006, 1615-9306.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10406 Analytical chemistry
Stát vydavatele Německo
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.535
Kód RIV RIV/00216224:14310/06:00016304
Organizační jednotka Přírodovědecká fakulta
UT WoS 000239574100011
Klíčová slova anglicky capillary isoelectric focusing;neutralization reaction boundary;proteins;fractionation;injected large volume sample
Štítky capillary isoelectric focusing, fractionation, injected large volume sample, neutralization reaction boundary, proteins
Příznaky Recenzováno
Změnil Změnil: doc. RNDr. Bc. Jiří Pazourek, Ph.D., učo 2356. Změněno: 26. 1. 2007 12:55.
Anotace
Mass spectrometry (MS) is being increasingly used for analysis of proteome complex samples. To remove matrix interferences and to concentrate analytes prior to a MS measurement, sample preparation is often necessary. A useful method for this purpose is Carrier Ampholyte Free-Isoelectric Focusing (CAF-IEF). In this paper CAF-IEF of ampholytes was performed on a commercial apparatus EA101 (Villa Labeco, Slovakia) equipped with a specially made column for samples of large volumes (up to 0.5 ml). A new continuous mode without voltage interruption or electrolyte replacement was developed. In this mode, a low molecular pI marker (PIM 7.4), low concentrations of myoglobin and insulin (16 mg/l), respectively, were concentrated, and then 5-ul fractions collected for off-line analyses. The total time of focusing was 66 minutes. Concentration of PIM 7.4 in the fractions was increased up to 75 times (determined by UV-VIS spectrometry). Concentration in fractions was increased up to 30 times for myoglobin and 10 times for insulin, respectively.
Anotace česky
Mass spectrometry (MS) is being increasingly used for analysis of proteome complex samples. To remove matrix interferences and to concentrate analytes prior to a MS measurement, sample preparation is often necessary. A useful method for this purpose is Carrier Ampholyte Free-Isoelectric Focusing (CAF-IEF). In this paper CAF-IEF of ampholytes was performed on a commercial apparatus EA101 (Villa Labeco, Slovakia) equipped with a specially made column for samples of large volumes (up to 0.5 ml). A new continuous mode without voltage interruption or electrolyte replacement was developed. In this mode, a low molecular pI marker (PIM 7.4), low concentrations of myoglobin and insulin (16 mg/l), respectively, were concentrated, and then 5-ul fractions collected for off-line analyses. The total time of focusing was 66 minutes. Concentration of PIM 7.4 in the fractions was increased up to 75 times (determined by UV-VIS spectrometry). Concentration in fractions was increased up to 30 times for myoglobin and 10 times for insulin, respectively.
Návaznosti
GA305/03/1100, projekt VaVNázev: Syntéza a studium neuroprotektivních peptidů odvozených od humaninu
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