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@article{709404,
author = {Tillinger, Andrej and Nováková, Marie and Pavlovičová, M. and Lacinová, Ľubica and Zatovičová, L. and Pastoreková, Sylvia and Križanová, Olga and Kvetňanský, Richard},
article_number = {4},
keywords = {PNMT; cardiomyocytes; chemical sympathectomy; immobilization stress},
language = {eng},
issn = {1025-3890},
journal = {Stress},
title = {Modulation by 6-hydroxydopamine of expression of the phenylethanolamine N-methyltransferase (PNMT) gene in the rat heart during immobilization stress},
volume = {9},
year = {2006}
}
TY - JOUR
ID - 709404
AU - Tillinger, Andrej - Nováková, Marie - Pavlovičová, M. - Lacinová, Ľubica - Zatovičová, L. - Pastoreková, Sylvia - Križanová, Olga - Kvetňanský, Richard
PY - 2006
TI - Modulation by 6-hydroxydopamine of expression of the phenylethanolamine N-methyltransferase (PNMT) gene in the rat heart during immobilization stress
JF - Stress
VL - 9
IS - 4
SP - 207-213
EP - 207-213
PB - UK Informa Ltd.
SN - 10253890
KW - PNMT
KW - cardiomyocytes
KW - chemical sympathectomy
KW - immobilization stress
N2 - Phenylethanolamine N-methyltransferase (PNMT) is the final enzyme in the catecholamine synthesizing cascade that converts noradrenaline (NA) to adrenaline (Adr). Both of these catecholamines are physiologically important hormones and neurotransmitters in mammals with profound influence on the activity of the cardiovascular system. Although PNMT activity and gene expression have been reported in the neonatal and also adult rat heart, little is known about the identity of the cells expressing PNMT mRNA. In this study, we have shown that besides PNMT in neuronal and intrinsic cardiac cells, this enzyme is expressed also in rat cardiomyocytes, as shown by immunofluorescence in isolated cardiomyocytes. To determine which cells in the heart more sensitively show stress-induced changes in PMNT mRNA expression, we performed chemical sympathectomy by administration of 6-hydroxydopamine (6-OHDA), which destroys catecholaminergic terminals. We determined PMNT mRNA levels in the left atria and ventricles of control and stressed rats. In the rats treated with 6-OHDA, PNMT mRNA levels were not changed under normal, physiological conditions compared to vehicle treated rats. Similar results we observed on isolated cardiomyocytes from control and 6-OHDA treated rats. However, 6-OHDA treatment prevented immobilization-induced increase in PNMT mRNA expression. The results allow us to propose that in the heart, the immobilization-induced increase in PNMT gene expression is probably not in cardiomyocytes, but in neuronal cells.
ER -
TILLINGER, Andrej, Marie NOVÁKOVÁ, M. PAVLOVIČOVÁ, Ľubica LACINOVÁ, L. ZATOVIČOVÁ, Sylvia PASTOREKOVÁ, Olga KRIŽANOVÁ and Richard KVETŇANSKÝ. Modulation by 6-hydroxydopamine of expression of the phenylethanolamine N-methyltransferase (PNMT) gene in the rat heart during immobilization stress. \textit{Stress}. UK Informa Ltd., 2006, vol.~9, No~4, p.~207-213. ISSN~1025-3890.
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