HASOŇ, Stanislav and Vladimír VETTERL. Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming. Analytical Chemistry. USA: AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS, 2006, 78/2006, No 14, p. 5179-5183. ISSN 0003-2700. |
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@article{710547, author = {Hasoň, Stanislav and Vetterl, Vladimír}, article_location = {USA}, article_number = {14}, keywords = {cathodic stripping voltammetry; graphite electrodes; DNA hybridization; magnetic nanoparticles; oligodeoxynucleotides;}, language = {eng}, issn = {0003-2700}, journal = {Analytical Chemistry}, title = {Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming}, volume = {78/2006}, year = {2006} }
TY - JOUR ID - 710547 AU - Hasoň, Stanislav - Vetterl, Vladimír PY - 2006 TI - Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming JF - Analytical Chemistry VL - 78/2006 IS - 14 SP - 5179-5183 EP - 5179-5183 PB - AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS SN - 00032700 KW - cathodic stripping voltammetry KW - graphite electrodes KW - DNA hybridization KW - magnetic nanoparticles KW - oligodeoxynucleotides; N2 - A simple, cost-effective design for amplifying oligodeoxynucleotide (ODN) sensing, in microliter ODN volumes containing copper ions, by solution streaming ( bubbling)is presented. The inert gas streaming ( bubbling) at a constant pressure of 0.04 bar drives the motion of a 30 microliter ODN droplet containing a three-electrode circuit ( inverted drop microcell), and in the presence of copper ions offers an similar to 50-times improvement in the detection of ODN samples. The detection of ODNs at the carbon paste electrode is based on the enhancement of the oxidation peaks of purine bases ( adenine and guanine) by the anodic stripping of the electrochemically accumulated complex of Cu(I) with purine base residues of acid hydrolyzed ODN samples ( Cu(I)-ahODN complex). ER -
HASOŇ, Stanislav and Vladimír VETTERL. Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming. \textit{Analytical Chemistry}. USA: AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS, 2006, 78/2006, No~14, p.~5179-5183. ISSN~0003-2700.
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