FOJT, Lukáš, Luděk STRAŠÁK and Vladimír VETTERL. Effect of electromagnetic fields on the denitrification activity of Paracoccus denitrificans. Bioelectrochemistry. The Netherlands: Elsevier, 2007, vol. 70, No 1, p. 91-95. ISSN 1567-5394.
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Basic information
Original name Effect of electromagnetic fields on the denitrification activity of Paracoccus denitrificans
Name in Czech Vliv elektromagnetických polí na denitrifikační aktivitu Paracoccus denitrificans.
Authors FOJT, Lukáš (203 Czech Republic), Luděk STRAŠÁK (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor).
Edition Bioelectrochemistry, The Netherlands, Elsevier, 2007, 1567-5394.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10610 Biophysics
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.992
RIV identification code RIV/00216224:14110/07:00019359
Organization unit Faculty of Medicine
UT WoS 000243708600017
Keywords in English low-frequency electromagnetic field; denitrification; Paracoccus denitrificans; graphite electrode; CFU number;
Tags CFU number, denitrification, Graphite electrode, Low-frequency electromagnetic field, Paracoccus denitrificans
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Vladimír Vetterl, DrSc., učo 1954. Changed: 30/6/2008 13:55.
Abstract
Enzymatic activity (denitrification) of Paracoccus denitrificans was estimated electrochemically by reduction of duroquinone (DQ). Graphite electrodes covered with whole bacterial cells behind a dialysis membrane were used for measurement. P. denitrificans reduce nitrate and/or nitrite under anaerobic conditions to nitrogen gas. DQ acts as an electron mediator. After donation of the electrons to the respiratory system of the bacteria, produced DQ is reduced to durohydroquinone on the electrode surface electrocatalytically. P. denitrificans were exposed to low-frequency magnetic field (10 mT, 50 Hz) for 24 min. In comparison with the control samples, the reduction peak that represents denitrification activity of the cells decreased significantly after magnetic field exposure. The decrease of the peak current was about 20%. The CFU-colony forming units-method was used to estimate the number of surviving bacteria. After 24 min exposure of 10 mT magnetic field P. denitrificans culture on electrode indicates 21% bacterial death.
Abstract (in Czech)
Enzymatická aktivita (denitrifikace) Paracoccus denitrificans byla sledována eletrochemicky redukcí durochinonu.
Links
IAA4004404, research and development projectName: Interakce biopolymerů s ligandy a detekce jejich konformačních změn na fázových rozhraních pomocí elektrochemických a optických metod
Investor: Academy of Sciences of the Czech Republic, Interaction of biopolymers with ligands and detection of their conformation changes at interfaces by electrochemical and optical methods.
IBS5004107, research and development projectName: Aplikace biofyzikálních metod v biotechnologické a klinické praxi
Investor: Academy of Sciences of the Czech Republic, Application of biophysical methods in biotechnological and clinical praxis
1M0528, research and development projectName: Stomatologické výzkumné centrum
Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre
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