Double elimination of oligonucleotides

MIKELOVÁ, Radka, Libuše TRNKOVÁ and František JELEN. Double elimination of oligonucleotides. In Sborník příspěvků VII. Pracovního setkání fyzikálních chemiků a elektrochemiků. Brno: Masarykova universita, 2007, p. 99-101. ISBN 9788021042353.

Basic information

• Original name: Double elimination of oligonucleotides
• Name in Czech: Dvojnásobná eliminace oligonukleotidů
• Authors: MIKELOVÁ, Radka (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and František JELEN (203 Czech Republic).
• Edition: Brno, Sborník příspěvků VII. Pracovního setkání fyzikálních chemiků a elektrochemiků, p. 99-101, 3 pp. 2007.
• Publisher: Masarykova universita

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10405 Electrochemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/07:00019364
• Organization unit: Faculty of Science
• ISBN: 9788021042353
• Keywords in English: Elimination voltammetry; Double elimination voltammetry;electrochemistry; Oligonucleotides
• Tags: double elimination voltammetry, electrochemistry, Elimination voltammetry, Oligonucleotides

Abstract

Ten years ago the theory of elimination voltammetry with linear scan (EVLS) was published and experimentally verified for reversible, quasi-reversible and irreversible electrode systems1-3. The EVLS can be considered a mathematical model of the transformation of current-potential curves capable of eliminating some selected current components, and conserving the other ones. This contribution is devoted to the EVLS transformation of irreversible current-potential curves of an adsorbed electroactive substance. The function eliminating charging and kinetic current components, and conserving the diffusion current component, yields specific, sensitive and well developed peak-couterpeak (p-cp) signal3-5. Theoretical value of ratio Ip/(Ip+Icp) is 0,40973,5,8. This signal was successfully employed in the analysis of nucleic acids and short homo- or hetero-deoxyoligonucleotides (ODNs) containing adenine (A) and cytosine (C)4-12.

Abstract (in Czech)

Double eliminace oligonukleotidů

Links

• IAA100040602, research and development project: Name: Nové přístupy v elektrochemické analýze nukleových kyselin a oligonukleotidů zaměřené na ultrasensitivní mikrodetekci DNA a detekci DNA hybridizace.

Investor: Academy of Sciences of the Czech Republic, New approaches in electrochemical analysis of nucleic acids and oligonucleotides aimed at a ultrasensitive microdetection of DNA and DNA hybridization

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)