Detailed Information on Publication Record
2007
A determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry
ADAM, Vojtěch, Soňa KŘÍŽKOVÁ, Ondřej ZÍTKA, Libuše TRNKOVÁ, J. PETRLOVÁ et. al.Basic information
Original name
A determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry
Name in Czech
Stanovení apo-metalothionein použitím adsorptivní přenosové rozpouštěcí techniky ve spojení s diferenční pulsní voltametrií
Authors
ADAM, Vojtěch (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), Ondřej ZÍTKA (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic), J. PETRLOVÁ (203 Czech Republic), Miroslava BEKLOVÁ (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)
Edition
Electroanalysis, Weinheim, Wiley.VCH Verlag, 2007, 1040-0397
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10405 Electrochemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.949
RIV identification code
RIV/00216224:14310/07:00019366
Organization unit
Faculty of Science
UT WoS
000244220700031
Keywords in English
apo-metallothionein; adsorptive transfer stripping technique; differential pulse voltammetry
Změněno: 20/6/2008 13:28, prof. RNDr. Libuše Trnková, CSc.
V originále
Links between metallothionein (MT), its structure and many biologically important pathways demonstrate the necessity of taking into account of studying the behavior of MTin different well defined mediums, which could help to model conditions in an organism easily. The main aim of this work was to prepare and determine apoMT by adsorptive transfer stripping technique (AdTS) in connection with differential pulse voltammetry (DPV). Particularly, we investigated the electrochemical behavior of MT measured on the surface of hanging mercury drop electrode in the presence of sodium chloride as supporting electrolyte using AdTS DPV with respect to study the effects of MT signals by different concentrations and pHs of the electrolyte. Then, we aimed at utilizing this technique to observe changes in MT which are dependent on using strong chelating compounds ethylendiamine N, N, N, N-tetraacetic acid (EDTA). Thanks to ability of EDTA to bind heavy metals from active center of enzymes and regulation proteins, we were able to prepare apoMT; that means, MT without any metal ion bound in its structure. Detection limit of apoMT at very short time of accumulation (tA 120 s) was 3 nM (20 ng/mL and/or 15 fmol in 5 mL drop; RSD 2 to 5%) estimated by dilution of the analyzed solution until the signal disappeared. In addition, it was possible to decrease detection limit by extending of time of accumulation of apoMT on the surface of HMDE. We were able to detect 30 pM of apoMT (200 pg/mL and/or 150 amol in 5 mL drop) at tA500 s.
In Czech
Stanovení apo metalothioneinu použitím adsorptivní přenosové rozpouštěcí techniky ve spojení s diferenční pulsní voltametrií
Links
| GP525/04/P132, research and development project |
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| IAA100040602, research and development project |
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| MSM0021622412, plan (intention) |
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