Detailed Information on Publication Record
2007
Integration of short-end injection procedure into EMMA methodology.
NĚMEC, Tomáš and Zdeněk GLATZBasic information
Original name
Integration of short-end injection procedure into EMMA methodology.
Name in Czech
Spojeni techniky dávkování z krátkého konce kapiláry s metodou EMMA
Authors
NĚMEC, Tomáš (203 Czech Republic) and Zdeněk GLATZ (203 Czech Republic, guarantor)
Edition
Journal of Chromatography A, Amsterdam, Elsevier, 2007, 0021-9673
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.641
RIV identification code
RIV/00216224:14310/07:00020172
Organization unit
Faculty of Science
UT WoS
000247855200012
Keywords in English
CE; EMMA substrate; inhibition; haloalkane dehalogenase
Tags
International impact, Reviewed
Změněno: 16/7/2008 14:59, Mgr. Petr Bureš
V originále
The possibility of integration of the short end injection mode in the EMMA methodology is demonstrated in this work on the kinetic studies of haloalkane dehalogenase and rhodanese enzymatic reactions. The essential validations of the EMMA methods combined with the short end and long end injection modes were performed first to confirm their accuracy. The qualitative and quantitative parameters of both approaches such as repetabilities of migration times and peak areas, limits of detection and correlation coefficients were in acceptable ranges. In addition estimated Michaelis constants for the corresponding substrate(s) were comparable being in accordance with previous literature data. Moreover the ping-pong reaction mechanism of rhodanese reaction was confirmed by means of both injection modes. This combination thus preserves the benefits of these instrumental approaches. Whereas the short-end injection procedure brought 5 6.5 times reduction of the analysis time and 2.5 4 times increase of the sensitivity, the EMMA methodology allowed full automatization of the assays while the whole kinetic studies needed only 20 ul of corresponding enzyme preparation.
In Czech
Byla integravána technika dávkování z krátkého konce kapiláry do metody EMMA, která přinesla nejenom zkrácení doby analýzy, ale rovněž zvýšení citlivosti detekce.
Links
| GA203/06/0047, research and development project |
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| LC06023, research and development project |
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| MSM0021622413, plan (intention) |
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