HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats

JUŘICA, Jan, Miroslav DOSTÁLEK, Jiří KONEČNÝ, Zdeněk GLATZ, Eva HADAŠOVÁ and Josef TOMANDL. HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2007, vol. 852, 1-2, p. 571-577. ISSN 1570-0232.

Basic information

• Original name: HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats
• Name in Czech: HPLC stanovení midazolamu a jeho tří hydroxy-metabolitů v perfuzním médiu a plazmě potkanů
• Authors: JUŘICA, Jan (203 Czech Republic, belonging to the institution), Miroslav DOSTÁLEK (203 Czech Republic), Jiří KONEČNÝ (203 Czech Republic, belonging to the institution), Zdeněk GLATZ (203 Czech Republic, belonging to the institution), Eva HADAŠOVÁ (203 Czech Republic, belonging to the institution) and Josef TOMANDL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2007, 1570-0232.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.935
• RIV identification code: RIV/00216224:14310/07:00020174
• Organization unit: Faculty of Science
• UT WoS: 000247286700078
• Keywords in English: cytochrome P450; midazolam; 1'-hydroxymidazolam; 4-hydroxymidazolam; 1'4-dihydroxymidazolam; rat; perfusion; plasma; HPLC
• Tags: 1'-hydroxymidazolam, 1'4-dihydroxymidazolam, 4-hydroxymidazolam, cytochrome P450, HPLC, midazolam, perfusion, plasma, rat
• Tags: International impact, Reviewed

Abstract

A new, simple, rapid, sensitive, and repeatable isocratic reverse-phase HPLC method was developed and validated for simultaneous determination of midazolam and its main three hydroxylated metabolites, i.e. 1' hydroxymidazolam, 4-hydroxymidazolam, and 1',4-dihydroxymidazolam in rat liver perfusate and also plasma. Diazepam was used as an internal standard to ensure precision and accuracy of this method. This method is applicable for modeling and description of possible pharmacological interactions on rat (CYP3A1/2) or human (CYP3A4/5) cytochrome P450 enzymes.

Abstract (in Czech)

Byla vypracována HPLC metoad současného stanovení midazolamu a jeho hlavních tří hydroxylovaných metabolitů v perfuzním mediu a plazmě ptokanů. Metoda je využitelná při modelování a charakterizaci možných farmakologických interakcí isoformy CYP3A1/2 u potkanů nebo CYP3A4/5 u člověka.

Links

• GA203/06/0047, research and development project: Name: Využití kapilární elektroforézy pro studium metabolismu léčiv

Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies

• LC06023, research and development project: Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.