Detailed Information on Publication Record
2007
The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella
HYRŠL, Pavel, Ender BÜYÜKGÜZEL and Kemal BÜYÜKGÜZELBasic information
Original name
The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella
Name in Czech
Vliv oxidativního stresu indukovaného kyselinou boritou na antioxidační enzymy a přežívání Galleria mellonella
Authors
HYRŠL, Pavel (203 Czech Republic, guarantor), Ender BÜYÜKGÜZEL (792 Turkey) and Kemal BÜYÜKGÜZEL (792 Turkey)
Edition
Archives of Insect Biochemistry and Physiology, Wiley, 2007, 0739-4462
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30105 Physiology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.345
RIV identification code
RIV/00216224:14310/07:00031927
Organization unit
Faculty of Science
UT WoS
000248872200003
Keywords in English
Galleria mellonella; boric acid; antioxidant enzymes; hemolymph; fat body; malondialdehyde; survivorship
Tags
Tags
International impact, Reviewed
Změněno: 28/4/2011 15:51, doc. RNDr. Pavel Hyršl, Ph.D.
V originále
Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1250, or 2500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on diets with 156 and 620 ppm BA doses yielded increased SOD activity but 1250 and 2500 ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity, however they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1250 and 2500 ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. By contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management.
In Czech
Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1250, or 2500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on diets with 156 and 620 ppm BA doses yielded increased SOD activity but 1250 and 2500 ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity, however they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1250 and 2500 ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. By contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management.