Vinca alkaloid effect on microtubules of HeLa cells.

BEČVÁŘOVÁ, Pavlína, Jiřina ŠKORPÍKOVÁ, Roman JANISCH and Jindřich NOVÝ. Vinca alkaloid effect on microtubules of HeLa cells. Scripta Medica. Brno: Lékařská fakulta MU Brno, 2006, vol. 79, No 1, p. 19-34, 14 pp. ISSN 1211-3395.

Basic information

• Original name: Vinca alkaloid effect on microtubules of HeLa cells.
• Name in Czech: Účine vinca alkaloidů na mikrotubuly HeLa buněk.
• Authors: BEČVÁŘOVÁ, Pavlína (203 Czech Republic), Jiřina ŠKORPÍKOVÁ (203 Czech Republic), Roman JANISCH (203 Czech Republic, guarantor) and Jindřich NOVÝ (203 Czech Republic).
• Edition: Scripta Medica, Brno, Lékařská fakulta MU Brno, 2006, 1211-3395.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10610 Biophysics
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/06:00016405
• Organization unit: Faculty of Medicine
• Keywords in English: Cytoskeleton; Microtubule disruption; Cytoskeleton recovery; vincristine; vinblastine
• Tags: Cytoskeleton, Cytoskeleton recovery, Microtubule disruption, vinblastine, vincristine

Abstract

The study evaluated the effect of selected vinca alkaloids on the microtubule network of heLa cells and the recovery of its disruption. Model experiments were used for demonstration of the quantification of microtubule network changes induced by vinca alkaloids using computer-assisted image analysis. Differences in the arrangement of microtubules were assessed by means of a software for quantification of the cytoskeleton changes in cells treated with vincristine at a concentration of 0,020 g/l and in untreated control cells. The microtubule network recovery process in time intervals of 6, 7, 8, 9, and 10 hours was quantified after treatment with vincristine at a concentration of 0,020 g/l for 60 min using the software for quantification of the cytoskeleton changes.

Abstract (in Czech)

heLa buňky byly ovlivněny vinkristinem nebo vinblastinem o koncentraci od 0,002 do 0,8 g/l po dobu 60ti minut nebo vinkristinem o koncentraci 4,615 g/l a 9,230 g/l po dobu 60ti minut. Mikrotubuly byly detekovány metodou nepřímé imunofluorescence a poškození bylo stanoveno pomocí fluorescenčního mikroskopu. Změny v uspořádání mikrotubulů byly u buněk ovlivněných vinkristinem o koncentraci 0,020 g/l po dobu 60 min a u buněk neovlivněných hodnoceny pomocí softwaru pro kvantifikaci cytoskeletálních změn. Dále byl s použitím softwaru pro kvantifikaci změn cytoskeletu studován proces reparace mikrotubulů v časových intervalech 6, 7, 8, 9 a 10 hodin, po ovlivnění vinkristinem o koncentraci 0,020 g/l po dobu 60 minut.

Links

• GD301/03/H005, research and development project: Name: Molekulární a buněčná biologie v biomedicíně

Investor: Czech Science Foundation, Molecular and cell biology in biomedicine