Detailed Information on Publication Record
2007
Distinct patterns of histone methylation and acetylation in human interphase nuclei
SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Vladimír ULMAN, Petr MATULA, David SVOBODA et. al.Basic information
Original name
Distinct patterns of histone methylation and acetylation in human interphase nuclei
Name in Czech
Rozdílné paterny metylace a acetylace histonů v lidských interfázních jádrech
Authors
SKALNÍKOVÁ, Magdalena (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Vladimír ULMAN (203 Czech Republic, belonging to the institution), Petr MATULA (203 Czech Republic, belonging to the institution), David SVOBODA (203 Czech Republic, belonging to the institution), Andrea HARNIČAROVÁ (703 Slovakia, belonging to the institution), Michal KOZUBEK (203 Czech Republic, guarantor, belonging to the institution) and Stanislav KOZUBEK (203 Czech Republic, belonging to the institution)
Edition
Physiological Research, Prague, Czech Academy of Sciences, 2007, 0862-8408
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.505
RIV identification code
RIV/00216224:14330/07:00020185
Organization unit
Faculty of Informatics
UT WoS
000252358700017
Keywords in English
Histone methylation; acetylation; X chromosome; centromere; image analysis
Tags
International impact, Reviewed
Změněno: 20/6/2012 09:03, RNDr. Vladimír Ulman, Ph.D.
V originále
To study 3D nuclear distributions of epigenetic histone modifications such as H3(K9) acetylation, H3(K4) dimethylation, H3(K9) dimethylation, and H3(K27) trimethylation, and of histone methyl transferase Suv39H1, we used advanced image analysis methods, combined with Nipkow disk confocal microscopy. Total fluorescence intensity and distributions of fluorescently labelled proteins were analysed in formaldehyde-fixed interphase nuclei. Our data showed reduced fluorescent signals of H3(K9) acetylation and H3(K4) dimethylation (dime) at the nuclear periphery, while di-meH3(K9) was also abundant in chromatin regions closely associated with the nuclear envelope. Little overlapping (intermingling) was observed for di-meH3(K4) and H3(K27) trimethylation (tri-me), and for di-meH3(K9) and Suv39H1. The histone modifications studied were absent in the nucleolar compartment with an exception of H3(K9) dimethylation that was closely associated to perinucleolar regions which are formed by centromeres of acrocentric chromosomes. Using immunocytochemistry, no dimeH3( K4) but just dense di-meH3(K9) was found for the human acrocentric chromosomes 14 and 22. The active X chromosome was observed to be partially acetylated, while the inactive X was more condensed, located in a very peripheral part of the interphase nuclei, and lacked H3(K9) acetylation. Our results confirmed specific interphase patterns of histone modifications within the interphase nuclei as well as within their chromosome territories.
In Czech
Práce se zabývá různými "paterny" metylace a acetylace histonů v lidských interfázních jádrech
Links
| GP202/03/D033, research and development project |
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| LC535, research and development project |
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| MSM0021622419, plan (intention) |
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