The paper is focused on an application of microeconomic conception of economic efficiency in industrial enterprises in the Czech Republic. The basic relationship of economic efficiency measurement level by the help of economic efficiency index as a ratio of real and maximal efficiency was deduced on the basis of the neoclassic model of the firm. Through the use of an analogy was constructed business economic model of the firm, which comes from the neoclassic model and accounting data too. It made possible construction of an economic efficiency ratio in practice and furthermore to measure the level of an efficiency of sample industrial enterprises. The legitimacy of a procedure and results was tested by the mean of regression equations made by computer programme MATLAB.