MATIASOVICOVA, J., P. ADAMS, P.A. BARROW, Helena HRADECKÁ, M. MALCOVA, R. KARPISKOVA, Eva BUDINSKÁ and Lenka PILOUSOVÁ. Identification of putative ancestors of the multidrug-resistant Salmonella enterica serovar typhimurium DT104 clone harboring the Salmonella genomic island 1. Archives of microbiology. 2007, vol. 187, No 5, p. 415-424. ISSN 0302-8933.
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Basic information
Original name Identification of putative ancestors of the multidrug-resistant Salmonella enterica serovar typhimurium DT104 clone harboring the Salmonella genomic island 1
Name in Czech Identification of putative ancestors of the multidrug-resistant Salmonella enterica serovar typhimurium DT104 clone harboring the Salmonella genomic island 1
Authors MATIASOVICOVA, J. (203 Czech Republic), P. ADAMS (826 United Kingdom of Great Britain and Northern Ireland), P.A. BARROW (826 United Kingdom of Great Britain and Northern Ireland), Helena HRADECKÁ (203 Czech Republic, guarantor, belonging to the institution), M. MALCOVA (203 Czech Republic), R. KARPISKOVA (203 Czech Republic), Eva BUDINSKÁ (703 Slovakia, belonging to the institution) and Lenka PILOUSOVÁ (203 Czech Republic, belonging to the institution).
Edition Archives of microbiology, 2007, 0302-8933.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.838
RIV identification code RIV/00216224:14310/07:00050770
Organization unit Faculty of Science
UT WoS 000245669200008
Keywords in English Salmonella typhimurium DT104 SGI1 Microarray genomotyping Prophage Allantoin
Tags International impact, Reviewed
Changed by Changed by: Mgr. Eva Budinská, Ph.D., učo 40822. Changed: 10/4/2012 11:04.
Abstract
The origin of multidrug-resistant Salmonella enterica serovar typhimurium (S. typhimurium) harboring the Salmonella Genomic Island 1 (SGI1), which was detected for the first time in the mid-1980s is unknown. In this study, we performed microarray genomotyping of four multidrug-resistant SGI1 positive strains and found that unlike the S. typhimurium LT2 strain, the multidrug-resistant strains lacked genes STM0517-0529 allowing the utilization of allantoin as a sole nitrogen source. We extended this observation by PCR screening of additional 120 S. typhimurium field strains and found that this locus was absent in all SGI1 positive and also in 24% of SGI1 negative strains, which were proposed to be the original recipients of SGI1. To prove this hypothesis, we compared the STM0517-0529 negative strains (with or without the SGI1) by PFGE and PCR prophage typing and found that 8 out of 11 of the SGI1 negative strains and 17 out of 22 SGI1 positive strains were of identical PFGE pattern and PCR prophage pattern, while this specific pattern was never observed among STM0517-0529 positive strains. We therefore propose that a lineage of the S. typhimurium DT104 sensitive strain first lost the ability to metabolize allantoin and then acquired SGI1.
Abstract (in Czech)
The origin of multidrug-resistant Salmonella enterica serovar typhimurium (S. typhimurium) harboring the Salmonella Genomic Island 1 (SGI1), which was detected for the first time in the mid-1980s is unknown. In this study, we performed microarray genomotyping of four multidrug-resistant SGI1 positive strains and found that unlike the S. typhimurium LT2 strain, the multidrug-resistant strains lacked genes STM0517-0529 allowing the utilization of allantoin as a sole nitrogen source. We extended this observation by PCR screening of additional 120 S. typhimurium field strains and found that this locus was absent in all SGI1 positive and also in 24% of SGI1 negative strains, which were proposed to be the original recipients of SGI1. To prove this hypothesis, we compared the STM0517-0529 negative strains (with or without the SGI1) by PFGE and PCR prophage typing and found that 8 out of 11 of the SGI1 negative strains and 17 out of 22 SGI1 positive strains were of identical PFGE pattern and PCR prophage pattern, while this specific pattern was never observed among STM0517-0529 positive strains. We therefore propose that a lineage of the S. typhimurium DT104 sensitive strain first lost the ability to metabolize allantoin and then acquired SGI1.
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