The mink as an animal model for Pseudomonas aeruginosa
adhesion: binding of the bacterial lectins (PA-IL and PA-IIL)
to neoglycoproteins and to sections of pancreas and lung
tissues from healthy mink

J 2007

The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink

KIRKEBY, Svend, Michaela WIMMEROVÁ, Dennis MOE and Axel K. HANSEN

Basic information

Original name

The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink

Name in Czech

Morče jako živočišný model pro adhezi bakterie Pseudomonas aeruginosa: vazba bakteriálních lektinů (PA-IL a PA-IIL)

Authors

KIRKEBY, Svend (208 Denmark), Michaela WIMMEROVÁ (203 Czech Republic, guarantor), Dennis MOE (208 Denmark) and Axel K. HANSEN (208 Denmark)

Edition

Microbes and Infection, Paris, Elsevier, 2007, 1286-4579

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.523

RIV identification code

RIV/00216224:14310/07:00020257

Organization unit

Faculty of Science

UT WoS

000247107600003

Keywords in English

pseudomonas aeruginosa; lectin;cystic fibrosis

Abstract

ORIG CZ

V originále

Lung infection with Pseudomonas aeruginosa, leading to chronic lung disease with impaired function, is the major course of morbidity and mortality among cystic fibrosis patients. The bacterium produces two lectins that bind to a-D-galactose (PA-IL) and L-fucose (PA-IIL), respectively, and lectinecarbohydrate interactions may be involved in microbial pathogenicity by creating bacterial adherence to epithelial and endothelial cells. An ideal animal model for P. aeruginosa infection has until now not been established, but the mink seems to be the only animal that has been reported to develop spontaneous P. aeruginosa infections in the airways. Since cystic fibrosis also severely may affect pancreatic function, we incubated sections from mink lungs and pancreas with a medium containing Pseudomonas lectins in order to detect in situ binding of the bacterial lectins. In the lungs, both lectins adhered to seromucinous glands located in the submucosa of the larger bronchi. Additionally, PA-IL reacted with the capillaries in the alveolar walls and with the small blood vessels forming the vasa vasorum around the larger vessels, while PAIIL marked the goblet cells in the bronchial surface epithelium. In the pancreas, both lectins bound to the epithelium in the excretory ducts, and additionally, PA-IL strongly stained the pancreatic capillaries while PA-IIL staining was noticed in the apical part of acinar cells in the exocrine part of the gland while no lectin reaction could be recorded in the endocrine cells. Judging from the results in the present paper the mink should be considered a suitable model to study P. aeruginosa adherence.

In Czech

Morče jako živočišný model pro adhezi bakterie Pseudomonas aeruginosa: vazba bakteriálních lektinů (PA-IL a PA-IIL)

Links

GA303/06/0570, research and development project

Name: Strukturně-funkční studie lektinů a adhezinů patogenních mikroorganismů

Investor: Czech Science Foundation, Structure-function studies on lectins and adhesins from microbial patogens

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

KIRKEBY, Svend, Michaela WIMMEROVÁ, Dennis MOE and Axel K. HANSEN. The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink. Microbes and Infection. Paris: Elsevier, 2007, vol. 9, No 5, p. 566-573. ISSN 1286-4579.

@article{718507,
   author = {Kirkeby, Svend and Wimmerová, Michaela and Moe, Dennis and Hansen, Axel K.},
   article_location = {Paris},
   article_number = {5},
   keywords = {pseudomonas aeruginosa; lectin;cystic fibrosis},
   language = {eng},
   issn = {1286-4579},
   journal = {Microbes and Infection},
   title = {The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink},
   volume = {9},
   year = {2007}
}

TY  - JOUR
ID  - 718507
AU  - Kirkeby, Svend - Wimmerová, Michaela - Moe, Dennis - Hansen, Axel K.
PY  - 2007
TI  - The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink
JF  - Microbes and Infection
VL  - 9
IS  - 5
SP  - 566-573
EP  - 566-573
PB  - Elsevier
SN  - 12864579
KW  - pseudomonas aeruginosa
KW  - lectin;cystic fibrosis
N2  - Lung infection with Pseudomonas aeruginosa, leading to chronic lung disease with impaired function, is the major course of morbidity and mortality among cystic fibrosis patients. The bacterium produces two lectins that bind to a-D-galactose (PA-IL) and L-fucose (PA-IIL), respectively, and lectinecarbohydrate interactions may be involved in microbial pathogenicity by creating bacterial adherence to epithelial and endothelial cells. An ideal animal model for P. aeruginosa infection has until now not been established, but the mink seems to be the only animal that has been reported to develop spontaneous P. aeruginosa infections in the airways. Since cystic fibrosis also severely may affect pancreatic function, we incubated sections from mink lungs and pancreas with a medium containing Pseudomonas lectins in order to detect in situ binding of the bacterial lectins. In the lungs, both lectins adhered to seromucinous glands located in the submucosa of the larger bronchi. Additionally, PA-IL reacted with the capillaries in the alveolar walls and with the small blood vessels forming the vasa vasorum around the larger vessels, while PAIIL marked the goblet cells in the bronchial surface epithelium. In the pancreas, both lectins bound to the epithelium in the excretory ducts, and additionally, PA-IL strongly stained the pancreatic capillaries while PA-IIL staining was noticed in the apical part of acinar cells in the exocrine part of the gland while no lectin reaction could be recorded in the endocrine cells. Judging from the results in the present paper the mink should be considered a suitable model to study P. aeruginosa adherence.
ER  -

KIRKEBY, Svend, Michaela WIMMEROVÁ, Dennis MOE and Axel K. HANSEN. The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink. \textit{Microbes and Infection}. Paris: Elsevier, 2007, vol.~9, No~5, p.~566-573. ISSN~1286-4579.

Detailed Information on Publication Record