VYSKOČILOVÁ, Martina, Andrea ŠIMKOVÁ and Jean-François MARTIN. Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae. Molecular Ecology Notes. Blackwell Publishing, 2007, vol. 7, No 6, p. 1150-1154. ISSN 1471-8278. |
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@article{718608, author = {Vyskočilová, Martina and Šimková, Andrea and Martin, JeanandFrançois}, article_number = {6}, keywords = {Leuciscus; Cyprinidae; microsatellites; primers; cross-amplification}, language = {eng}, issn = {1471-8278}, journal = {Molecular Ecology Notes}, title = {Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae.}, volume = {7}, year = {2007} }
TY - JOUR ID - 718608 AU - Vyskočilová, Martina - Šimková, Andrea - Martin, Jean-François PY - 2007 TI - Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae. JF - Molecular Ecology Notes VL - 7 IS - 6 SP - 1150-1154 EP - 1150-1154 PB - Blackwell Publishing SN - 14718278 KW - Leuciscus KW - Cyprinidae KW - microsatellites KW - primers KW - cross-amplification N2 - Thirteen polymorphic microsatellites were isolated from Leuciscus cephalus, a widespread cyprinid species with great ecological tolerance. Together with the cross-species amplification of six additional loci originally published for three cyprinid fish species, we optimized a multiplex panel for L. cephalus allowing the genotyping of 19 polymorphic loci. Number of alleles and heterozygosity per locus in a sample of 20 fish individuals ranged from two to sixteen and from 0.05 to 0.90, respectively. These primers will be useful in determining the population structure of L. cephalus. In addition, successful cross-amplification was obtained for several species of Cyprinidae. ER -
VYSKOČILOVÁ, Martina, Andrea ŠIMKOVÁ and Jean-Fran$\backslash$c cois MARTIN. Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae. \textit{Molecular Ecology Notes}. Blackwell Publishing, 2007, vol.~7, No~6, p.~1150-1154. ISSN~1471-8278.
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