Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae.

VYSKOČILOVÁ, Martina, Andrea ŠIMKOVÁ and Jean-François MARTIN. Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae. Molecular Ecology Notes. Blackwell Publishing, 2007, vol. 7, No 6, p. 1150-1154. ISSN 1471-8278.

Basic information

• Original name: Isolation and characterization of microsatellites in Leuciscus cephalus (Cypriniformes, Cyprinidae) and cross-species amplification within the family Cyprinidae.
• Name in Czech: Izolace a charakterizace nových mikrosatelitů u Leuciscus cephalus (Cypriniformes, Cyprinidae) a cross-species amplifikace v čeledi Cyprinidae.
• Authors: VYSKOČILOVÁ, Martina (203 Czech Republic, guarantor, belonging to the institution), Andrea ŠIMKOVÁ (703 Slovakia, belonging to the institution) and Jean-François MARTIN (250 France).
• Edition: Molecular Ecology Notes, Blackwell Publishing, 2007, 1471-8278.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.257
• RIV identification code: RIV/00216224:14310/07:00020260
• Organization unit: Faculty of Science
• UT WoS: 000250647700065
• Keywords in English: Leuciscus; Cyprinidae; microsatellites; primers; cross-amplification
• Tags: cross-amplification, Cyprinidae, Leuciscus, microsatellites, primers

Abstract

Thirteen polymorphic microsatellites were isolated from Leuciscus cephalus, a widespread cyprinid species with great ecological tolerance. Together with the cross-species amplification of six additional loci originally published for three cyprinid fish species, we optimized a multiplex panel for L. cephalus allowing the genotyping of 19 polymorphic loci. Number of alleles and heterozygosity per locus in a sample of 20 fish individuals ranged from two to sixteen and from 0.05 to 0.90, respectively. These primers will be useful in determining the population structure of L. cephalus. In addition, successful cross-amplification was obtained for several species of Cyprinidae.

Abstract (in Czech)

Pro Leuciscus cephalus bylo izolováno a charakterizováno 13 polymorfních mikrosatelitů, úspěšně bylo testováno 6 mikrosatelitových markerů původně popsaných pro jiné druhy z čeledi Cyprinidae. Dále bylo všech 19 mikrosatelitů použito pro cross-species amplifikaci u několika daších druhů ryb z čeledi Cyprinidae.

Links

• GA524/04/1128, research and development project: Name: MHC třída IIB genů evropských kaprovitých ryb: jejich genetická variabilita a evoluce ve vztahu k životní strategii hostitelů a parazitismu

Investor: Czech Science Foundation, MHC class IIB genes of European cyprinid fish: their genetic variability and evolution in relation to the host life-history traits and parasitism

• LC522, research and development project: Name: ICHTYOPARAZITOLOGIE - centrum základního výzkumu

Investor: Ministry of Education, Youth and Sports of the CR, Ichtyoparazitology - the center of basic research

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time