VYSKOČILOVÁ, Martina, Markéta ONDRAČKOVÁ, Andrea ŠIMKOVÁ and Jean-François MARTIN. Isolation and characterization of microsatellites in Neogobius kessleri (Perciformes, Gobiidae) and cross-species amplification within the family Gobiidae. Molecular Ecology Notes. Blackwell Publishing, 2007, Neuveden, No 7, p. 701-704. ISSN 1471-8278.
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Basic information
Original name Isolation and characterization of microsatellites in Neogobius kessleri (Perciformes, Gobiidae) and cross-species amplification within the family Gobiidae
Name in Czech Izolace a charekterizace nových mikrosatelitů pro Neogobius kessleri (Perciformes, Gobiidae) a cross-species amplifikace v čeledi Gobiidae
Authors VYSKOČILOVÁ, Martina (203 Czech Republic, guarantor, belonging to the institution), Markéta ONDRAČKOVÁ (703 Slovakia, belonging to the institution), Andrea ŠIMKOVÁ (703 Slovakia, belonging to the institution) and Jean-François MARTIN (250 France).
Edition Molecular Ecology Notes, Blackwell Publishing, 2007, 1471-8278.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.257
RIV identification code RIV/00216224:14310/07:00020261
Organization unit Faculty of Science
UT WoS 000247758100045
Keywords in English Neogobius; Proterorhinus; Gobiidae; microsatellites; primers; cross-amplification
Tags cross-amplification, Gobiidae, microsatellites, Neogobius, primers, Proterorhinus
Changed by Changed by: prof. RNDr. Andrea Vetešníková Šimková, PhD., učo 24570. Changed: 31/3/2011 12:53.
Abstract
Fourteen polymorphic microsatellites were isolated from Neogobius kessleri, a benthic fish of Ponto-Caspian origin which has been recently introduced into the Middle and Upper Danube River. Number of alleles and heterozygosity per locus in a sample of 32 fish individuals ranged from two to four and from 0.13 to 0.75, respectively. These primers will be useful in determining the population structure of N. kessleri. In addition, successful cross-amplification was obtained for four related species, N. melanostomus, N. fluviatilis, N. gymnotrachelus and Proterorhinus marmoratus. These microsatellite loci may be useful for the evaluation of the origin of non-native goby populations.
Abstract (in Czech)
Pro Neogobius kessleri bylo izolováno a charakterizováno 14 polymorfních mikrosatelitů. Dále byly všechny mikrosatelity použity pro cross-species amplifikace u N. melanostomus, N. fluviatilis, N. gymnotrachelus and Proterorhinus marmoratus.
Links
GP524/05/P291, research and development projectName: Parazitismus a invazní druhy: vliv parazitárního napadení na biologii Neogobius kessleri v původní a nepůvodní oblasti rozšíření
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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