| VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL, K.J. KRAMER and René KIZEK. Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions. Biologica Plantarum. 2007, vol. 51, No 3, p. 551-555. ISSN 0006-3134. |
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@article{718616,
author = {Víteček, Jan and Petrlová, Jitka and Petřek, Jiří and Adam, Vojtěch and Havel, L. and Kramer, K.J. and Kizek, René},
article_number = {3},
keywords = {fluorimetric analysis; plant esterases; programmed cell death; cadmium(II) ions},
language = {eng},
issn = {0006-3134},
journal = {Biologica Plantarum},
title = {Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions},
volume = {51},
year = {2007}
}
TY - JOUR
ID - 718616
AU - Víteček, Jan - Petrlová, Jitka - Petřek, Jiří - Adam, Vojtěch - Havel, L. - Kramer, K.J. - Kizek, René
PY - 2007
TI - Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions
JF - Biologica Plantarum
VL - 51
IS - 3
SP - 551-555
EP - 551-555
SN - 00063134
KW - fluorimetric analysis
KW - plant esterases
KW - programmed cell death
KW - cadmium(II) ions
N2 - Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.
ER -
VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL, K.J. KRAMER and René KIZEK. Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions. \textit{Biologica Plantarum}. 2007, vol.~51, No~3, p.~551-555. ISSN~0006-3134.
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