Application of fluorimetric analysis of plant esterases to
study of programmed cell death and effects of cadmium(II) ions

J 2007

Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL et. al.

Basic information

Original name

Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

Name in Czech

Aplikace fluorimetrické analýzy rostlinných esteráz při studiu progamované buněčné smrti a vliv kademnatých iontů

Authors

VÍTEČEK, Jan (203 Czech Republic), Jitka PETRLOVÁ (203 Czech Republic), Jiří PETŘEK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), L. HAVEL (203 Czech Republic), K.J. KRAMER (840 United States of America) and René KIZEK (203 Czech Republic, guarantor)

Edition

Biologica Plantarum, 2007, 0006-3134

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.259

RIV identification code

RIV/00216224:14310/07:00020262

Organization unit

Faculty of Science

UT WoS

000247412400026

Keywords in English

fluorimetric analysis; plant esterases; programmed cell death; cadmium(II) ions

Abstract

ORIG CZ

V originále

Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.

In Czech

Aplikace fluorimetrické analýzy rostlinných esteráz při studiu progamované buněčné smrti a vliv kademnatých iontů

Links

GP525/04/P132, research and development project

Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

Citovat

VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL, K.J. KRAMER and René KIZEK. Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions. Biologica Plantarum. 2007, vol. 51, No 3, p. 551-555. ISSN 0006-3134.

@article{718616,
   author = {Víteček, Jan and Petrlová, Jitka and Petřek, Jiří and Adam, Vojtěch and Havel, L. and Kramer, K.J. and Kizek, René},
   article_number = {3},
   keywords = {fluorimetric analysis; plant esterases; programmed cell death; cadmium(II) ions},
   language = {eng},
   issn = {0006-3134},
   journal = {Biologica Plantarum},
   title = {Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions},
   volume = {51},
   year = {2007}
}

TY  - JOUR
ID  - 718616
AU  - Víteček, Jan - Petrlová, Jitka - Petřek, Jiří - Adam, Vojtěch - Havel, L. - Kramer, K.J. - Kizek, René
PY  - 2007
TI  - Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions
JF  - Biologica Plantarum
VL  - 51
IS  - 3
SP  - 551-555
EP  - 551-555
SN  - 00063134
KW  - fluorimetric analysis
KW  - plant esterases
KW  - programmed cell death
KW  - cadmium(II) ions
N2  - Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.
ER  -

VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL, K.J. KRAMER and René KIZEK. Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions. \textit{Biologica Plantarum}. 2007, vol.~51, No~3, p.~551-555. ISSN~0006-3134.

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