A sensor for investigating the interaction between biologically
important heavy metals and glutathione

J 2007

A sensor for investigating the interaction between biologically important heavy metals and glutathione

HUSKA, D., Ondřej ZÍTKA, Vojtěch ADAM, M. BEKLOVÁ, Soňa KŘÍŽKOVÁ et. al.

Basic information

Original name

A sensor for investigating the interaction between biologically important heavy metals and glutathione

Name in Czech

Senzor pro sledování interakcí mezi biologicky významnými těžkými kovy a glutathioneinem

Authors

HUSKA, D. (203 Czech Republic), Ondřej ZÍTKA (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), M. BEKLOVÁ (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), L. ZEMAN (203 Czech Republic), A. HORNA (276 Germany), L. HAVEL (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)

Edition

Czech J. Anim. Sci. 2007, 1212-1819

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.633

RIV identification code

RIV/00216224:14310/07:00020268

Organization unit

Faculty of Science

UT WoS

000244285800002

Keywords in English

sensor; interaction; biologically important heavy metals; glutathione

Abstract

ORIG CZ

V originále

Glutathione (GSH) is the water-soluble tripeptide playing a crucial role in number of cellular reactions including detoxification of heavy metals. Glutathione can be found both in oxidized (GSSG) and reduced (GSH) state. The aim of this work was to utilize the electrochemical methods for determining of glutathiones and for investigating interaction of GSH and cadmium, zinc, copper and nickel ions or food supplement for animal nutrition. It clearly follows from the results obtained that GSH and GSSG can be distinguished by cyclic voltammetry whereas the height of their current response is proportional to their concentration. Moreover, we were concerned with studying of these interactions by cyclic voltammetry. The markedly changes of GSH signal were observed and discussed. The highest decrease of GSH reductive signal cause cadmium ions followed by zinc, nickel and copper ones. Moreover we observed that reductive GSH signal gradually decreased (about 9 %) with increasing concentration of the feedstuff additive used in our experiments. In the conclusion, cyclic voltammetry offers a tool for studying of interaction of thiols with different substances such as heavy metals and/or feedstuff additives.

In Czech

Senzor pro sledování interakcí mezi biologicky významnými těžkými kovy a glutathioneinem

Links

GP525/04/P132, research and development project

Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

Citovat

HUSKA, D., Ondřej ZÍTKA, Vojtěch ADAM, M. BEKLOVÁ, Soňa KŘÍŽKOVÁ, L. ZEMAN, A. HORNA, L. HAVEL, Josef ZEHNÁLEK and René KIZEK. A sensor for investigating the interaction between biologically important heavy metals and glutathione. Czech J. Anim. Sci. 2007, vol. 52, No 2, p. 37-43, 6 pp. ISSN 1212-1819.

@article{718628,
   author = {Huska, D. and Zítka, Ondřej and Adam, Vojtěch and Beklová, M. and Křížková, Soňa and Zeman, L. and Horna, A. and Havel, L. and Zehnálek, Josef and Kizek, René},
   article_number = {2},
   keywords = {sensor; interaction; biologically important heavy metals; glutathione},
   language = {eng},
   issn = {1212-1819},
   journal = {Czech J. Anim. Sci.},
   title = {A sensor for investigating the interaction between biologically important heavy metals and glutathione},
   volume = {52},
   year = {2007}
}

TY  - JOUR
ID  - 718628
AU  - Huska, D. - Zítka, Ondřej - Adam, Vojtěch - Beklová, M. - Křížková, Soňa - Zeman, L. - Horna, A. - Havel, L. - Zehnálek, Josef - Kizek, René
PY  - 2007
TI  - A sensor for investigating the interaction between biologically important heavy metals and glutathione
JF  - Czech J. Anim. Sci.
VL  - 52
IS  - 2
SP  - 37-43
EP  - 37-43
SN  - 12121819
KW  - sensor
KW  - interaction
KW  - biologically important heavy metals
KW  - glutathione
N2  - Glutathione (GSH) is the water-soluble tripeptide playing a crucial role in number of cellular reactions including detoxification of heavy metals. Glutathione can be found both in oxidized (GSSG) and reduced (GSH) state. The aim of this work was to utilize the electrochemical methods for determining of glutathiones and for investigating interaction of GSH and cadmium, zinc, copper and nickel ions or food supplement for animal nutrition. It clearly follows from the results obtained that GSH and GSSG can be distinguished by cyclic voltammetry whereas the height of their current response is proportional to their concentration. Moreover, we were concerned with studying of these interactions by cyclic voltammetry. The markedly changes of GSH signal were observed and discussed. The highest decrease of GSH reductive signal cause cadmium ions followed by zinc, nickel and copper ones. Moreover we observed that reductive GSH signal gradually decreased (about 9 %) with increasing concentration of the feedstuff additive used in our experiments. In the conclusion, cyclic voltammetry offers a tool for studying of interaction of thiols with different substances such as heavy metals and/or feedstuff additives.
ER  -

HUSKA, D., Ondřej ZÍTKA, Vojtěch ADAM, M. BEKLOVÁ, Soňa KŘÍŽKOVÁ, L. ZEMAN, A. HORNA, L. HAVEL, Josef ZEHNÁLEK and René KIZEK. A sensor for investigating the interaction between biologically important heavy metals and glutathione. \textit{Czech J. Anim. Sci.}. 2007, vol.~52, No~2, p.~37-43, 6 pp. ISSN~1212-1819.

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