Bacteria profiling by MALDI-MS: The influence of sample preparation on distinguishing closely related Pseudomonas species

ŠEDO, Ondrej, Andrea VÁVROVÁ, Ludmila TVRZOVÁ and Zbyněk ZDRÁHAL. Bacteria profiling by MALDI-MS: The influence of sample preparation on distinguishing closely related Pseudomonas species. In Book of Abstracts and Program. 2007. ISBN 978-963-7067-14-3.

Basic information

Original name

Bacteria profiling by MALDI-MS: The influence of sample preparation on distinguishing closely related Pseudomonas species

Name in Czech

Charakterizace bakterií pomocí MALDI-MS: Vliv přípravy vzorku na rozlišení příbuzných členů rodu Pseudomonas

Authors

ŠEDO, Ondrej (203 Czech Republic, belonging to the institution), Andrea VÁVROVÁ (203 Czech Republic, belonging to the institution), Ludmila TVRZOVÁ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Book of Abstracts and Program, 2007

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Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Hungary

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/07:00022179

Organization unit

Faculty of Science

ISBN

978-963-7067-14-3

Keywords in English

bacteria profiling; MALDI MS; Pseudomonas

Tags

bacteria profiling, MALDI MS, Pseudomonas

Tags

International impact, Reviewed

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Abstract

V originále

In this work we examined numerous influences of particular sample preparation steps to observe whether their variation may affect the final output of data processing.Direct MALDI-MS analysis of bacteria extracts with subsequent evaluation using spectra libraries represents a new and rapid technique for microorganism identification. Numerous laboratories have developed approaches leading to successful distinguishing of desired bacteria strains; however, there are several differences in described sample treatment protocols, which might delimit their universality.The genus Pseudomonas (Prokaryotes, Proteobacteria, Gammaproteobacteria) can be an example of environmentally and clinically important genus with complicated and evolving taxonomy and problematically distinguishable species. The genus is phylogenetically very heterogeneous and comprises species ubiquitous in soil and water ecosystems and important as plant, animal and human pathogens.

In Czech

V práci byly sledovány význam jednotlivých kroků přípravy vzorků pro analýzu MALDI-MS s cílem zjistit jejich vliv na konečné výsledky statistické analýzy při rozlišování jednotlivých členů rodu Pseudomonas.

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Links

LC06034, research and development project	Name: Regulace morfogeneze rostlinných buněk a orgánů Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622413, plan (intention)	Name: Proteiny v metabolismu a při interakci organismů s prostředím Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
MSM0021622415, plan (intention)	Name: Molekulární podstata buněčných a tkáňových regulací Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations