Capillary isoelectric focusing of proteins and microorganisms
in dynamically modified fused silica with UV detection.

J 2006

Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection.

HORKÁ, Marie, Filip RŮŽIČKA, Jaroslav HORKÝ, Veronika HOLÁ, Karel ŠLAIS et. al.

Basic information

Original name

Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection.

Name in Czech

CIEF proteinů a mikroorganizmů v dynamicky modifikované křemičité kapiláře s UV detekcí.

Authors

HORKÁ, Marie (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, guarantor), Jaroslav HORKÝ (203 Czech Republic), Veronika HOLÁ (203 Czech Republic) and Karel ŠLAIS (203 Czech Republic)

Edition

Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2006, 1570-0232

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30300 3.3 Health sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.647

RIV identification code

RIV/00216224:14110/06:00016449

Organization unit

Faculty of Medicine

UT WoS

000240393200017

Keywords (in Czech)

kapilární izoelektrická fokusace; proteiny; mikroorganizmy; dynamická modifikace; UV detekce

Keywords in English

capillary isoelectric focusing; proteins; microorganisms; dynamically modified fused silica; UV detection.

Abstract

ORIG CZ

V originále

We suggest a method for the reproducible and efficient capillary isoelectric focusing of proteins and microorganisms in the pH gradient 3-10. The method involves the segmental injection of the simple ampholytes, the solution of the selected electrolytes, and the sample mixture of bioanalytes and carrier ampholytes to the fused silica capillaries dynamically modified by poly(ethylene glycol), PEG 4000, which is added to the catholyte, the anolyte and injected solutions. In order to receive the reproducible results, the capillaries were rinsed by the mixture of acetone/ethanol between analyses. For the tracing of the pH gradients the low-molecular-mass pI markers were used. The simple proteins and the mixed cultures of microorganisms, Saccharomyces cerevisiae CCM 8191, Escherichia coli CCM 3954, Candida albicans CCM 8180, Candida parapsilosis, Candida krusei, Staphylococcus aureus, Streptococcus agalactiae CCM 6187, Enterococcus faecalis CCM 4224, Staphylococcus epidermidis CCM 4418 and Stenotrophomonas maltophilia, were focused and separated by the method suggested. The minimum detectable number of microbial cells was 5x10(2) to 1x10(3) with on-column UV detection at 280 nm.

In Czech

Navržená CIEF metoda umožňuje citlivě a reprodukovatelně v gradientu pH 3-10 detekovat a identifikovat proteiny i mikroorganizmy a to jak z čistých kultur, tak ze směsí.

Links

IAA4031302, research and development project

Name: Využití rychlé elektroforetické separace s velmi citlivou fluorimetrickou detekcí pro identifikaci mikroorganismů

Investor: Academy of Sciences of the Czech Republic

Citovat

HORKÁ, Marie, Filip RŮŽIČKA, Jaroslav HORKÝ, Veronika HOLÁ and Karel ŠLAIS. Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2006, vol. 841, 1-2, p. 152-159. ISSN 1570-0232.

@article{719269,
   author = {Horká, Marie and Růžička, Filip and Horký, Jaroslav and Holá, Veronika and Šlais, Karel},
   article_location = {Amsterdam, The Netherlands},
   article_number = {1-2},
   keywords = {capillary isoelectric focusing; proteins; microorganisms; dynamically modified fused silica; UV detection.},
   language = {eng},
   issn = {1570-0232},
   journal = {Journal of Chromatography B},
   title = {Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection.},
   volume = {841},
   year = {2006}
}

TY  - JOUR
ID  - 719269
AU  - Horká, Marie - Růžička, Filip - Horký, Jaroslav - Holá, Veronika - Šlais, Karel
PY  - 2006
TI  - Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection.
JF  - Journal of Chromatography B
VL  - 841
IS  - 1-2
SP  - 152-159
EP  - 152-159
PB  - Elsevier
SN  - 15700232
KW  - capillary isoelectric focusing
KW  - proteins
KW  - microorganisms
KW  - dynamically modified fused silica
KW  - UV detection.
N2  - We suggest a method for the reproducible and efficient capillary isoelectric focusing of proteins and microorganisms in the pH gradient 3-10. The method involves the segmental injection of the simple ampholytes, the solution of the selected electrolytes, and the sample mixture of bioanalytes and carrier ampholytes to the fused silica capillaries dynamically modified by poly(ethylene glycol), PEG 4000, which is added to the catholyte, the anolyte and injected solutions. In order to receive the reproducible results, the capillaries were rinsed by the mixture of acetone/ethanol between analyses. For the tracing of the pH gradients the low-molecular-mass pI markers were used. The simple proteins and the mixed cultures of microorganisms, Saccharomyces cerevisiae CCM 8191, Escherichia coli CCM 3954, Candida albicans CCM 8180, Candida parapsilosis, Candida krusei, Staphylococcus aureus, Streptococcus agalactiae CCM 6187, Enterococcus faecalis CCM 4224, Staphylococcus epidermidis CCM 4418 and Stenotrophomonas maltophilia, were focused and separated by the method suggested. The minimum detectable number of microbial cells was 5x10(2) to 1x10(3) with on-column UV detection at 280 nm.
ER  -

HORKÁ, Marie, Filip RŮŽIČKA, Jaroslav HORKÝ, Veronika HOLÁ and Karel ŠLAIS. Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection. \textit{Journal of Chromatography B}. Amsterdam, The Netherlands: Elsevier, 2006, vol.~841, 1-2, p.~152-159. ISSN~1570-0232.

Detailed Information on Publication Record