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@article{719272,
author = {Horká, Marie and Růžička, Filip and Horký, Jaroslav and Holá, Veronika and Šlais, Karel},
article_location = {Washington, D.C., USA},
article_number = {24},
keywords = {capillary isoelectric focusing; fluorometric detection; proteins; microorganisms; poly(ethylene glycol) pyrenebutanoate.},
language = {eng},
issn = {0003-2700},
journal = {Analytical Chemistry},
title = {Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.},
volume = {78},
year = {2006}
}
TY - JOUR
ID - 719272
AU - Horká, Marie - Růžička, Filip - Horký, Jaroslav - Holá, Veronika - Šlais, Karel
PY - 2006
TI - Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.
JF - Analytical Chemistry
VL - 78
IS - 24
SP - 8438-8444
EP - 8438-8444
PB - American Chemical Society
SN - 00032700
KW - capillary isoelectric focusing
KW - fluorometric detection
KW - proteins
KW - microorganisms
KW - poly(ethylene glycol) pyrenebutanoate.
N2 - The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. The values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pI markers and were found comparable with pI of the native compounds. The mixed cultures of proteins and microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis CCM 4418, Proteus vulgaris, Enterococcus faecalis CCM 4224, and Stenotrophomonas maltophilia, the strains of the yeast cells, Candida albicans CCM 8180, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae were reproducibly focused and separated by the suggested technique. Using UV excitation for the on-column fluorometric detection, the minimum detectable amount was down to 10 cells injected on the separation capillary.
ER -
HORKÁ, Marie, Filip RŮŽIČKA, Jaroslav HORKÝ, Veronika HOLÁ and Karel ŠLAIS. Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2006, vol.~78, No~24, p.~8438-8444, 8 pp. ISSN~0003-2700.
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