| POHANKA, Miroslav, Ota PAVLIŠ and Petr SKLÁDAL. Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor. Sensors. Basel, Switzerland: MDPI, 2007, vol. 7, No 3, p. 341-353. ISSN 1424-8220. |
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@article{719461,
author = {Pohanka, Miroslav and Pavliš, Ota and Skládal, Petr},
article_location = {Basel, Switzerland},
article_number = {3},
keywords = {biosensor; quartz crystal microbalance; Francisella tularensis},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor},
url = {http://www.mdpi.org/sensors/papers/s7030341.pdf},
volume = {7},
year = {2007}
}
TY - JOUR
ID - 719461
AU - Pohanka, Miroslav - Pavliš, Ota - Skládal, Petr
PY - 2007
TI - Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor
JF - Sensors
VL - 7
IS - 3
SP - 341-353
EP - 341-353
PB - MDPI
SN - 14248220
KW - biosensor
KW - quartz crystal microbalance
KW - Francisella tularensis
UR - http://www.mdpi.org/sensors/papers/s7030341.pdf
N2 - Monoclonal antibodies with specificity against the Francisella tularensis outerlipopolysaccharide (LPS) membrane were prepared and characterized using the piezoelectric immunosensor with immobilized LPS antigen from F. tularensis. Signals obtained by the immunosensor were compared with ELISA and similar sensitivity was noticed. Signal of negative controls obtained using the biosensor was below 0.5% of the signal obtained for the selected specific antibody clone 4H3B9D3. Testing of cross reactivity based on the sensors with immobilized LPS from Escherichia coli and Bacillus subtilis confirmed selectivity of this antibody. Furthermore, the 4H3B9D3 antibody was successfully isotypized as IgM using the piezoelectric sensors with secondary antibodies. Kinetics parameters of antibody were evaluated in the flow-through arrangement. The kinetic rate constants for the antibody 4H3B9D3 were ka = (2.31 +- 0.20)x10^5 l mol-1s-1 (association) and kd = (0.0010 +- 0.00062) s-1 (dissociation) indicating very good affinity to the LPS antigen.
ER -
POHANKA, Miroslav, Ota PAVLIŠ and Petr SKLÁDAL. Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor. \textit{Sensors}. Basel, Switzerland: MDPI, 2007, vol.~7, No~3, p.~341-353. ISSN~1424-8220.
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