Rapid Characterization of Monoclonal Antibodies using the
Piezoelectric Immunosensor

J 2007

Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor

POHANKA, Miroslav, Ota PAVLIŠ and Petr SKLÁDAL

Basic information

Original name

Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor

Name in Czech

Rychlá charakterizace monoklonálních protilátek použitím piezoelektrického imunosensoru

Authors

POHANKA, Miroslav (203 Czech Republic), Ota PAVLIŠ (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, guarantor)

Edition

Sensors, Basel, Switzerland, MDPI, 2007, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.573

RIV identification code

RIV/00216224:14310/07:00022210

Organization unit

Faculty of Science

UT WoS

000245395500007

Keywords in English

biosensor; quartz crystal microbalance; Francisella tularensis

Abstract

ORIG CZ

V originále

Monoclonal antibodies with specificity against the Francisella tularensis outerlipopolysaccharide (LPS) membrane were prepared and characterized using the piezoelectric immunosensor with immobilized LPS antigen from F. tularensis. Signals obtained by the immunosensor were compared with ELISA and similar sensitivity was noticed. Signal of negative controls obtained using the biosensor was below 0.5% of the signal obtained for the selected specific antibody clone 4H3B9D3. Testing of cross reactivity based on the sensors with immobilized LPS from Escherichia coli and Bacillus subtilis confirmed selectivity of this antibody. Furthermore, the 4H3B9D3 antibody was successfully isotypized as IgM using the piezoelectric sensors with secondary antibodies. Kinetics parameters of antibody were evaluated in the flow-through arrangement. The kinetic rate constants for the antibody 4H3B9D3 were ka = (2.31 +- 0.20)x10^5 l mol-1s-1 (association) and kd = (0.0010 +- 0.00062) s-1 (dissociation) indicating very good affinity to the LPS antigen.

In Czech

Byly hledány mAb specifické proti lipopolysacharidové složce bakterie Francisella tularensis pomocí piezoelektrického imunosensoru, nalezený klon byl kineticky charakterizován.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

POHANKA, Miroslav, Ota PAVLIŠ and Petr SKLÁDAL. Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor. Sensors. Basel, Switzerland: MDPI, 2007, vol. 7, No 3, p. 341-353. ISSN 1424-8220.

@article{719461,
   author = {Pohanka, Miroslav and Pavliš, Ota and Skládal, Petr},
   article_location = {Basel, Switzerland},
   article_number = {3},
   keywords = {biosensor; quartz crystal microbalance; Francisella tularensis},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor},
   url = {http://www.mdpi.org/sensors/papers/s7030341.pdf},
   volume = {7},
   year = {2007}
}

TY  - JOUR
ID  - 719461
AU  - Pohanka, Miroslav - Pavliš, Ota - Skládal, Petr
PY  - 2007
TI  - Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor
JF  - Sensors
VL  - 7
IS  - 3
SP  - 341-353
EP  - 341-353
PB  - MDPI
SN  - 14248220
KW  - biosensor
KW  - quartz crystal microbalance
KW  - Francisella tularensis
UR  - http://www.mdpi.org/sensors/papers/s7030341.pdf
N2  - Monoclonal antibodies with specificity against the Francisella tularensis outerlipopolysaccharide (LPS) membrane were prepared and characterized using the piezoelectric immunosensor with immobilized LPS antigen from F. tularensis. Signals obtained by the immunosensor were compared with ELISA and similar sensitivity was noticed. Signal of negative controls obtained using the biosensor was below 0.5% of the signal obtained for the selected specific antibody clone 4H3B9D3. Testing of cross reactivity based on the sensors with immobilized LPS from Escherichia coli and Bacillus subtilis confirmed selectivity of this antibody. Furthermore, the 4H3B9D3 antibody was successfully isotypized as IgM using the piezoelectric sensors with secondary antibodies. Kinetics parameters of antibody were evaluated in the flow-through arrangement. The kinetic rate constants for the antibody 4H3B9D3 were ka = (2.31 +- 0.20)x10^5 l mol-1s-1 (association) and kd = (0.0010 +- 0.00062) s-1 (dissociation) indicating very good affinity to the LPS antigen.
ER  -

POHANKA, Miroslav, Ota PAVLIŠ and Petr SKLÁDAL. Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor. \textit{Sensors}. Basel, Switzerland: MDPI, 2007, vol.~7, No~3, p.~341-353. ISSN~1424-8220.

Detailed Information on Publication Record