The occurrence of Ixodes ricinus ticks was observed in the suburban locality of Brno Pisárky (South Moravia, Czech Republic) from 1996 to 2002. Statistical tests divided the activity of ticks into three periods during the year. The curve of seasonality had two peaks with maximum in May and August, with a significantly larger number of specimens collected during this period compared to other months. The number of larvae, males and females was not significantly different, but the occurrence of nymphs was significantly greater. Except for the year 2000 there were no statistically significant differences in tick abundance. Tick activity was not dependent on humidity but did vary directly with temperature.