Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection
| KLEPÁRNÍK, Martin and Josef TOMANDL. Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection. In HPLC 2007. Abstract Book. Belgium: I.O.P.M.S. vzw, 2007, p. 909-909. |
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| Basic information | |
|---|---|
| Original name | Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection |
| Name in Czech | Stanovení asymetrického dimethylargininu v lidské plazmě pomocí HPLC s coulometrickou detekcí |
| Authors | KLEPÁRNÍK, Martin and Josef TOMANDL. |
| Edition | Belgium, HPLC 2007. Abstract Book. p. 909-909, 1 pp. 2007. |
| Publisher | I.O.P.M.S. vzw |
| Other information | |
|---|---|
| Original language | English |
| Type of outcome | Proceedings paper |
| Field of Study | 10406 Analytical chemistry |
| Country of publisher | Belgium |
| Confidentiality degree | is not subject to a state or trade secret |
| WWW | 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques - HPLC 2007 |
| Organization unit | Faculty of Medicine |
| Keywords in English | asymmetric dimethylarginine; HPLC; coularray detection |
| Tags | asymmetric dimethylarginine, coularray detection, HPLC |
| Tags | International impact, Reviewed |
| Changed by | Changed by: doc. RNDr. Josef Tomandl, Ph.D., učo 47. Changed: 26/9/2007 17:32. |
| Abstract |
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| Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection. |
| Abstract (in Czech) |
|---|
| Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection. |
| Links | |
|---|---|
| GA203/06/0047, research and development project | Name: Využití kapilární elektroforézy pro studium metabolismu léčiv |
| Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies | |
| LC06023, research and development project | Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie |
| Investor: Ministry of Education, Youth and Sports of the CR | |
| MSM0021622402, plan (intention) | Name: Časná diagnostika a léčba kardiovaskulárních chorob |
| Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases | |
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