D 2007

Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection

KLEPÁRNÍK, Martin and Josef TOMANDL

Basic information

Original name

Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection

Name in Czech

Stanovení asymetrického dimethylargininu v lidské plazmě pomocí HPLC s coulometrickou detekcí

Authors

KLEPÁRNÍK, Martin and Josef TOMANDL

Edition

Belgium, HPLC 2007. Abstract Book. p. 909-909, 1 pp. 2007

Publisher

I.O.P.M.S. vzw

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Belgium

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

31st International Symposium on High Performance Liquid Phase Separations and Related Techniques - HPLC 2007

Organization unit

Faculty of Medicine

Keywords in English

asymmetric dimethylarginine; HPLC; coularray detection

Tags

asymmetric dimethylarginine, coularray detection, HPLC

Tags

International impact, Reviewed
Změněno: 26/9/2007 17:32, doc. RNDr. Josef Tomandl, Ph.D.

Abstract

ORIG CZ

V originále

Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection.

In Czech

Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection.

Links

GA203/06/0047, research and development project
Name: Využití kapilární elektroforézy pro studium metabolismu léčiv
Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies
LC06023, research and development project
Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie
Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622402, plan (intention)
Name: Časná diagnostika a léčba kardiovaskulárních chorob
Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases
Displayed: 14/11/2024 02:11