Detailed Information on Publication Record
2007
HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats
JUŘICA, Jan, Martin KLEPÁRNÍK, Lucia ZAHRADNÍKOVÁ, Ondřej ZENDULKA, Josef TOMANDL et. al.Basic information
Original name
HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats
Name in Czech
HPLC-ED stanovení diklofenaku a jeho hydroxylovanýého metabolitu jako markeru metabolické aktivity CYP2C6 u potkanů
Authors
Edition
Belgium, HPLC 2007. Abstract Book. p. 723-723, 1 pp. 2007
Publisher
I.O.P.M.S. vzw
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10406 Analytical chemistry
Country of publisher
Belgium
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Organization unit
Faculty of Medicine
Keywords in English
HPLC; electrochemical detection; diclofenac; rat; cytochrome P450; CYP2C6
Tags
International impact
Změněno: 16/1/2008 09:05, doc. PharmDr. Jan Juřica, Ph.D.
V originále
Diclofenac belongs to the group of nonsteroidal antiinflammatory drugs. Presented method allows to determine concentration of diclofenac and its metabolite 4-hydroxydiclofenac in rat liver perfusate. The isolated rat liver was perfused with Williams medium E, the marker was administered as a bolus. The samples were collected in the 30th, 60th and 120th minute after the administration. Phenacetine was used as an internal standard (I.S.). After an addition of I.S. and pH adjustment, the analytes were extracted into diethyl ether and dried out under a gentle stream of nitrogen. The evaporated organic extract was dissolved in a mobile phase and analyzed on column Luna (2) C18 using coularray detection. The coulometric detection provides better sensitivity and limits of detection comparing to the conventional HPLC-UV methods. This method is suitable for modeling and description of possible pharmacological interactions on isoform CYP2C6.
In Czech
Prezentovaná HPLC-EC metoda umožňuje stanovit dikofenak a jeho metabolit 4-hydroxydiklofenak v jaterním perfuzátu s velmi vysokou přesností a selektivitou ve srovnání s konvenčním HPLC-UV stanovením. Metoda je použitelná při sledování fenotypu CYP2C6/11 u potkana.
Links
| GA203/06/0047, research and development project |
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| LC06023, research and development project |
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| MSM0021622404, plan (intention) |
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