HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats

JUŘICA, Jan, Martin KLEPÁRNÍK, Lucia ZAHRADNÍKOVÁ, Ondřej ZENDULKA and Josef TOMANDL. HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats. In HPLC 2007. Abstract Book. Belgium: I.O.P.M.S. vzw, 2007, p. 723-723.

Basic information

• Original name: HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats
• Name in Czech: HPLC-ED stanovení diklofenaku a jeho hydroxylovanýého metabolitu jako markeru metabolické aktivity CYP2C6 u potkanů
• Authors: JUŘICA, Jan, Martin KLEPÁRNÍK, Lucia ZAHRADNÍKOVÁ, Ondřej ZENDULKA and Josef TOMANDL.
• Edition: Belgium, HPLC 2007. Abstract Book. p. 723-723, 1 pp. 2007.
• Publisher: I.O.P.M.S. vzw

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Belgium
• Confidentiality degree: is not subject to a state or trade secret
• WWW: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques - HPLC 2007
• Organization unit: Faculty of Medicine
• Keywords in English: HPLC; electrochemical detection; diclofenac; rat; cytochrome P450; CYP2C6
• Tags: CYP2C6, cytochrome P450, diclofenac, electrochemical detection, HPLC, rat
• Tags: International impact

Abstract

Diclofenac belongs to the group of nonsteroidal antiinflammatory drugs. Presented method allows to determine concentration of diclofenac and its metabolite 4-hydroxydiclofenac in rat liver perfusate. The isolated rat liver was perfused with Williams medium E, the marker was administered as a bolus. The samples were collected in the 30th, 60th and 120th minute after the administration. Phenacetine was used as an internal standard (I.S.). After an addition of I.S. and pH adjustment, the analytes were extracted into diethyl ether and dried out under a gentle stream of nitrogen. The evaporated organic extract was dissolved in a mobile phase and analyzed on column Luna (2) C18 using coularray detection. The coulometric detection provides better sensitivity and limits of detection comparing to the conventional HPLC-UV methods. This method is suitable for modeling and description of possible pharmacological interactions on isoform CYP2C6.

Abstract (in Czech)

Prezentovaná HPLC-EC metoda umožňuje stanovit dikofenak a jeho metabolit 4-hydroxydiklofenak v jaterním perfuzátu s velmi vysokou přesností a selektivitou ve srovnání s konvenčním HPLC-UV stanovením. Metoda je použitelná při sledování fenotypu CYP2C6/11 u potkana.

Links

• GA203/06/0047, research and development project: Name: Využití kapilární elektroforézy pro studium metabolismu léčiv

Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies

• LC06023, research and development project: Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.