TETUROVÁ, Kateřina, Jana ŘEPKOVÁ, Pavel LÍZAL and Antonín DREISEITL. Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers. In Cereal science and technology for feeding ten billion people: genomics era and beyond. 2006th ed. Lleida, Spain: University of Lleida, Spain, 2006, p. 179.
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Basic information
Original name Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers
Name in Czech Identifikace genů odolnosti k padlí travnímu u nového zdroje Hordeum vulgare ssp. spontaneum pomocí DNA markerů
Authors TETUROVÁ, Kateřina (203 Czech Republic), Jana ŘEPKOVÁ (203 Czech Republic, guarantor), Pavel LÍZAL (203 Czech Republic) and Antonín DREISEITL (203 Czech Republic).
Edition 2006. vyd. Lleida, Spain, Cereal science and technology for feeding ten billion people: genomics era and beyond, p. 179-179, 2006.
Publisher University of Lleida, Spain
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Spain
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/06:00016463
Organization unit Faculty of Science
Keywords in English Hordeum vulgare; barley; powdery mildew; DNA markers; resistance
Tags barley, DNA markers, Hordeum vulgare, powdery mildew, resistance
Tags International impact
Changed by Changed by: Ing. Kateřina Teturová, Ph.D., učo 151559. Changed: 9/7/2007 14:48.
Abstract
An accession of wild barley (Hordeum vulgare ssp. spontaneum) carrying a newly identified resistance to powdery mildew (Blumeria graminis f. sp. hordei) was studied to localize the resistance genes on barley genetic map. Both genetic and molecular analyses were performed in the F2 population of the cross between the winter barley cultivar Tiffany and the resistant accession PI466197. The genetic analysis showed that the resistance is determined by two independent dominant genes and that one of the genes is in or in very close linkage with the Mla locus. DNA markers from on-line databases and literature were used for the localization of resistance genes. The susceptible F2 plants were screened to identify the markers linked with the resistance genes. Data from F2 plants of all genotypes were analysed in the MapManager QTX program to confirm the linkage and to assign the order and distances between the markers. The molecular analysis revealed a highly significant linkage with the markers Bmac0213 and MGB402 on chromosome 1H and Bmac0134, MWG878 and cMWG682 on chromosome 2H. The prospect of the work is to find more tightly linked DNA markers so that breeders could use them for marker assisted selection.
Abstract (in Czech)
Studium bylo zaměřeno na genetický popis nedávno získaného zdroje odolnosti k padlí travnímu PI466197. Genetická analýza prokázala determinaci odolnosti dvěma geny. Mikrosatelitní DNA markery byly využity k identifikaci obou genů odolnosti prostřednictvím jejich chromozomální lokalizace.
Links
GA522/03/0112, research and development projectName: Lokalizace genů odolnosti k Blumeria graminis f. sp. hordei v nově zjištěných zdrojích Hordeum vulgare ssp. spontaneum pomocí molekulárních markerů
GD204/05/H505, research and development projectName: Vývojová genetika rostlin
Investor: Czech Science Foundation, Developmental and Evolutionary Genetics of Plants
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