Identification of powdery mildew resistance genes in a new
accession of Hordeum vulgare ssp. spontaneum using DNA markers

D 2006

Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers

TETUROVÁ, Kateřina, Jana ŘEPKOVÁ, Pavel LÍZAL and Antonín DREISEITL

Basic information

Original name

Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers

Name in Czech

Identifikace genů odolnosti k padlí travnímu u nového zdroje Hordeum vulgare ssp. spontaneum pomocí DNA markerů

Authors

TETUROVÁ, Kateřina (203 Czech Republic), Jana ŘEPKOVÁ (203 Czech Republic, guarantor), Pavel LÍZAL (203 Czech Republic) and Antonín DREISEITL (203 Czech Republic)

Edition

2006. vyd. Lleida, Spain, Cereal science and technology for feeding ten billion people: genomics era and beyond, p. 179-179, 2006

Publisher

University of Lleida, Spain

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Spain

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/06:00016463

Organization unit

Faculty of Science

Keywords in English

Hordeum vulgare; barley; powdery mildew; DNA markers; resistance

Abstract

ORIG CZ

V originále

An accession of wild barley (Hordeum vulgare ssp. spontaneum) carrying a newly identified resistance to powdery mildew (Blumeria graminis f. sp. hordei) was studied to localize the resistance genes on barley genetic map. Both genetic and molecular analyses were performed in the F2 population of the cross between the winter barley cultivar Tiffany and the resistant accession PI466197. The genetic analysis showed that the resistance is determined by two independent dominant genes and that one of the genes is in or in very close linkage with the Mla locus. DNA markers from on-line databases and literature were used for the localization of resistance genes. The susceptible F2 plants were screened to identify the markers linked with the resistance genes. Data from F2 plants of all genotypes were analysed in the MapManager QTX program to confirm the linkage and to assign the order and distances between the markers. The molecular analysis revealed a highly significant linkage with the markers Bmac0213 and MGB402 on chromosome 1H and Bmac0134, MWG878 and cMWG682 on chromosome 2H. The prospect of the work is to find more tightly linked DNA markers so that breeders could use them for marker assisted selection.

In Czech

Studium bylo zaměřeno na genetický popis nedávno získaného zdroje odolnosti k padlí travnímu PI466197. Genetická analýza prokázala determinaci odolnosti dvěma geny. Mikrosatelitní DNA markery byly využity k identifikaci obou genů odolnosti prostřednictvím jejich chromozomální lokalizace.

Links

GA522/03/0112, research and development project

Name: Lokalizace genů odolnosti k Blumeria graminis f. sp. hordei v nově zjištěných zdrojích Hordeum vulgare ssp. spontaneum pomocí molekulárních markerů

GD204/05/H505, research and development project

Name: Vývojová genetika rostlin

Investor: Czech Science Foundation, Developmental and Evolutionary Genetics of Plants

Citovat

TETUROVÁ, Kateřina, Jana ŘEPKOVÁ, Pavel LÍZAL and Antonín DREISEITL. Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers. In Cereal science and technology for feeding ten billion people: genomics era and beyond. 2006th ed. Lleida, Spain: University of Lleida, Spain, 2006, p. 179.

@inproceedings{720331,
   author = {Teturová, Kateřina and Řepková, Jana and Lízal, Pavel and Dreiseitl, Antonín},
   address = {Lleida, Spain},
   booktitle = {Cereal science and technology for feeding ten billion people: genomics era and beyond},
   edition = {2006},
   keywords = {Hordeum vulgare; barley; powdery mildew; DNA markers; resistance},
   language = {eng},
   location = {Lleida, Spain},
   pages = {179-179},
   publisher = {University of Lleida, Spain},
   title = {Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers},
   year = {2006}
}

TY  - JOUR
ID  - 720331
AU  - Teturová, Kateřina - Řepková, Jana - Lízal, Pavel - Dreiseitl, Antonín
PY  - 2006
TI  - Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers
PB  - University of Lleida, Spain
CY  - Lleida, Spain
KW  - Hordeum vulgare
KW  - barley
KW  - powdery mildew
KW  - DNA markers
KW  - resistance
N2  - An accession of wild barley (Hordeum vulgare ssp. spontaneum) carrying a newly identified resistance to powdery mildew (Blumeria graminis f. sp. hordei) was studied to localize the resistance genes on barley genetic map. Both genetic and molecular analyses were performed in the F2 population of the cross between the winter barley cultivar Tiffany and the resistant accession PI466197. The genetic analysis showed that the resistance is determined by two independent dominant genes and that one of the genes is in or in very close linkage with the Mla locus. DNA markers from on-line databases and literature were used for the localization of resistance genes. The susceptible F2 plants were screened to identify the markers linked with the resistance genes. Data from F2 plants of all genotypes were analysed in the MapManager QTX program to confirm the linkage and to assign the order and distances between the markers. The molecular analysis revealed a highly significant linkage with the markers Bmac0213 and MGB402 on chromosome 1H and Bmac0134, MWG878 and cMWG682 on chromosome 2H. The prospect of the work is to find more tightly linked DNA markers so that breeders could use them for marker assisted selection.
ER  -

TETUROVÁ, Kateřina, Jana ŘEPKOVÁ, Pavel LÍZAL and Antonín DREISEITL. Identification of powdery mildew resistance genes in a new accession of Hordeum vulgare ssp. spontaneum using DNA markers. In \textit{Cereal science and technology for feeding ten billion people: genomics era and beyond}. 2006th ed. Lleida, Spain: University of Lleida, Spain, 2006, p.~179.

Detailed Information on Publication Record