Assessment of retinoic activity in fly ash and air samples from polluted areas using P19/A15 cell line

NOVÁK, Jiří, Martin BENÍŠEK, Tereza ŠTĚPÁNKOVÁ, Luděk BLÁHA, John Paul GIESY and Klára HILSCHEROVÁ. Assessment of retinoic activity in fly ash and air samples from polluted areas using P19/A15 cell line. In SETAC Europe 17th Annual Meeting -Abstract Book. 2007.

Basic information

• Original name: Assessment of retinoic activity in fly ash and air samples from polluted areas using P19/A15 cell line
• Name in Czech: Stanovení retinoidní aktivity popílku a vzorků vzduchu ze znečištěných lokalit za použití linie P19/A15
• Authors: NOVÁK, Jiří (203 Czech Republic, guarantor, belonging to the institution), Martin BENÍŠEK (203 Czech Republic, belonging to the institution), Tereza ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Luděk BLÁHA (203 Czech Republic, belonging to the institution), John Paul GIESY (840 United States of America) and Klára HILSCHEROVÁ (203 Czech Republic, belonging to the institution).
• Edition: SETAC Europe 17th Annual Meeting -Abstract Book, 2007.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30304 Public and environmental health
• Country of publisher: Portugal
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/07:00020343
• Organization unit: Faculty of Science
• Keywords in English: endocrine disruption; retinoid; complex samples
• Tags: complex samples, endocrine disruption, retinoid
• Tags: International impact

Abstract

Contamination in the environment has been described to induce malformations in amphibians. This fact could be linked with anti/retinoic action of the contaminants because retinoids control morphogenesis by affecting processes such as differentiation or apoptosis. In vitro model for evaluation of anti/retinoic action of xenobiotics was developed. It is based on murine cell line P19, which retains a pluripotent character and its differentiation is regulated mainly by retinoids. In the derived cell line P19/A15, the retinoic response element was linked with luciferase gene enabling the study of retinoid signaling. We tested air samples extracts from two areas in the vicinity of chemical factories producing different types of pollutants, extracts from fly ash from municipal incinerator and reference sample from background locality. Important activities were observed in both gas and particulate fractions of multiple air samples and in fly ash samples. The obtained results were compared with TEQs of each sample acquired by H4IIE.luc reporter gene bioassay. This research was supported by the projects GAČR 525/05/P160 and INCHEMBIOL 0021622412.

Abstract (in Czech)

Využití linie P19-A15 pro hodnocení toxicity látek narušujících retinoidní signálování

Links

• GP525/05/P160, research and development project: Name: In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů

Investor: Czech Science Foundation, In vitro models for study of endocrine disruption - effects of traditional and new types of persistent organic pollutants

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)