| JANOŠEK, Jaroslav, Michal BITTNER, Klára HILSCHEROVÁ, Luděk BLÁHA, J.P. GIESY and Ivan HOLOUBEK. AhR-mediated and antiestrogenic activity of humic substances. Chemosphere. Oxford, UK: Elsevier Science Ltd., 2007, vol. 67, No 6, p. 1096-1101. ISSN 0045-6535. |
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@article{720348,
author = {Janošek, Jaroslav and Bittner, Michal and Hilscherová, Klára and Bláha, Luděk and Giesy, J.P. and Holoubek, Ivan},
article_location = {Oxford, UK},
article_number = {6},
keywords = {Ah receptor;AhR-mediated activity;Antiestrogenic effects;Endocrine disruption},
language = {eng},
issn = {0045-6535},
journal = {Chemosphere},
title = {AhR-mediated and antiestrogenic activity of humic substances},
volume = {67},
year = {2007}
}
TY - JOUR
ID - 720348
AU - Janošek, Jaroslav - Bittner, Michal - Hilscherová, Klára - Bláha, Luděk - Giesy, J.P. - Holoubek, Ivan
PY - 2007
TI - AhR-mediated and antiestrogenic activity of humic substances
JF - Chemosphere
VL - 67
IS - 6
SP - 1096-1101
EP - 1096-1101
PB - Elsevier Science Ltd.
SN - 00456535
KW - Ah receptor;AhR-mediated activity;Antiestrogenic effects;Endocrine disruption
N2 - Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 x 10-8-7.4 x 10-8. Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists. Contribution of persistent organic compounds to the observed effects was ruled out by H2SO4 treatment. Eight out of twelve HS elicited significant antiestrogenic effects with IC50 ranging from 40 to 164 mg l-1. The possible explanations of the antiestrogenic effect include sorption of 17-[beta]-estradiol (E2) on HS, changes in membrane permeability for E2 or another specific mechanism.
ER -
JANOŠEK, Jaroslav, Michal BITTNER, Klára HILSCHEROVÁ, Luděk BLÁHA, J.P. GIESY and Ivan HOLOUBEK. AhR-mediated and antiestrogenic activity of humic substances. \textit{Chemosphere}. Oxford, UK: Elsevier Science Ltd., 2007, vol.~67, No~6, p.~1096-1101. ISSN~0045-6535.
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