Detailed Information on Publication Record
2007
AhR-mediated and antiestrogenic activity of humic substances
JANOŠEK, Jaroslav, Michal BITTNER, Klára HILSCHEROVÁ, Luděk BLÁHA, J.P. GIESY et. al.Basic information
Original name
AhR-mediated and antiestrogenic activity of humic substances
Name in Czech
AhR-zprostředkovaná a antiestrogenní aktivita huminových látek
Authors
JANOŠEK, Jaroslav (203 Czech Republic), Michal BITTNER (203 Czech Republic), Klára HILSCHEROVÁ (203 Czech Republic, guarantor), Luděk BLÁHA (203 Czech Republic), J.P. GIESY (840 United States of America) and Ivan HOLOUBEK (203 Czech Republic)
Edition
Chemosphere, Oxford, UK, Elsevier Science Ltd. 2007, 0045-6535
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30304 Public and environmental health
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.739
RIV identification code
RIV/00216224:14310/07:00020344
Organization unit
Faculty of Science
UT WoS
000245846700005
Keywords in English
Ah receptor;AhR-mediated activity;Antiestrogenic effects;Endocrine disruption
Změněno: 18/3/2010 10:16, prof. RNDr. Luděk Bláha, Ph.D.
V originále
Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 x 10-8-7.4 x 10-8. Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists. Contribution of persistent organic compounds to the observed effects was ruled out by H2SO4 treatment. Eight out of twelve HS elicited significant antiestrogenic effects with IC50 ranging from 40 to 164 mg l-1. The possible explanations of the antiestrogenic effect include sorption of 17-[beta]-estradiol (E2) on HS, changes in membrane permeability for E2 or another specific mechanism.
In Czech
doplnit
Links
| GP525/05/P160, research and development project |
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| MSM0021622412, plan (intention) |
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