Lignans of Schisandra chinensis: In situ extraction from cell
cultures and effect on cancer cells

D 2007

Lignans of Schisandra chinensis: In situ extraction from cell cultures and effect on cancer cells

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Zdenka SLUNSKÁ, Iva SLANINOVÁ, Jiří SLANINA et. al.

Basic information

Original name

Lignans of Schisandra chinensis: In situ extraction from cell cultures and effect on cancer cells

Name in Czech

Lignany Schisandra chinensis: In situ extrakce z buněčných kultur a efekt na nádorové buňky

Authors

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Zdenka SLUNSKÁ, Iva SLANINOVÁ and Jiří SLANINA

Edition

1. vyd. Bratislava, Synthetic and natural compounds in cancer therapy and prevention, p. 65-65, 2007

Publisher

Mind and Health, SR

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

ISBN

978-80-969663-2-5

Keywords in English

lignans; Schisandra; cancer

Abstract

ORIG CZ

V originále

S. chinensis cultures derived from immature zygotic embryo were developed on Murashige and Skoog and VW5 medium. Embryogenic culture was established on the medium containing thidiazuron, 2,4dichlorophenoxyacetic acid and benzylaminopurine. An analytical method based on HPLC was used for the determination of six lignans in plant cell cultures. We have explored the possibility of increasing the lignan release to medium by addition of two neutral polymeric resins (Amberlite XAD2 and XAD7) to the cell cultures. The polymeric resins not only notably enlarged portions of extracellular lignans, but also significantly enhanced production of total lignans. Amberlite XAD2 increased production of deoxyschizandrin, whereas Amberlite XAD7 enhanced the amount of wuweizisu C. Lignans adsorbed on polymeric resins can be easily recovered and purified without destruction of the cells. Lignans were also tested for their cytotoxicity on HeLa cells, A 431 human squamous cell epithelioma and human skin fibroblast. The nonpolar lignan gomisin N was found to be more cytotoxic than other lignans tested.

In Czech

Lignany Schisandra chinensis: In situ extrakce z buněčných kultur a efekt na nádorové buňky byl studován.

Links

GA522/07/0995, research and development project

Name: Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis

Investor: Czech Science Foundation

Citovat

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Zdenka SLUNSKÁ, Iva SLANINOVÁ and Jiří SLANINA. Lignans of Schisandra chinensis: In situ extraction from cell cultures and effect on cancer cells. In Synthetic and natural compounds in cancer therapy and prevention. 1st ed. Bratislava: Mind and Health, SR, 2007, p. 65. ISBN 978-80-969663-2-5.

@inproceedings{720473,
   author = {Březinová, Lenka and Vlašínová, Helena and Slunská, Zdenka and Slaninová, Iva and Slanina, Jiří},
   address = {Bratislava},
   booktitle = {Synthetic and natural compounds in cancer therapy and prevention},
   edition = {1},
   keywords = {lignans; Schisandra; cancer},
   language = {eng},
   location = {Bratislava},
   isbn = {978-80-969663-2-5},
   pages = {65-65},
   publisher = {Mind and Health, SR},
   title = {Lignans of Schisandra chinensis: In situ extraction from cell cultures and effect on cancer cells},
   year = {2007}
}

TY  - JOUR
ID  - 720473
AU  - Březinová, Lenka - Vlašínová, Helena - Slunská, Zdenka - Slaninová, Iva - Slanina, Jiří
PY  - 2007
TI  - Lignans of Schisandra chinensis: In situ extraction from cell cultures and effect on cancer cells
PB  - Mind and Health, SR
CY  - Bratislava
SN  - 9788096966325
KW  - lignans
KW  - Schisandra
KW  - cancer
N2  - S. chinensis cultures derived from immature zygotic embryo were developed on Murashige and Skoog and VW5 medium. Embryogenic culture was established on the medium containing thidiazuron, 2,4dichlorophenoxyacetic acid and benzylaminopurine. An analytical method based on HPLC was used for the determination of six lignans in plant cell cultures. We have explored the possibility of increasing the lignan release to medium by addition of two neutral polymeric resins (Amberlite XAD2 and XAD7) to the cell cultures. The polymeric resins not only notably enlarged portions of extracellular lignans, but also significantly enhanced production of total lignans. Amberlite XAD2 increased production of deoxyschizandrin, whereas Amberlite XAD7 enhanced the amount of wuweizisu C. Lignans adsorbed on polymeric resins can be easily recovered and purified without destruction of the cells. Lignans were also tested for their cytotoxicity on HeLa cells, A 431 human squamous cell epithelioma and human skin fibroblast. The nonpolar lignan gomisin N was found to be more cytotoxic than other lignans tested.
ER  -

BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Zdenka SLUNSKÁ, Iva SLANINOVÁ and Jiří SLANINA. Lignans of Schisandra chinensis: In situ extraction from cell cultures and effect on cancer cells. In \textit{Synthetic and natural compounds in cancer therapy and prevention}. 1st ed. Bratislava: Mind and Health, SR, 2007, p.~65. ISBN~978-80-969663-2-5.

Detailed Information on Publication Record