Cytotoxic and colour properties of quaternary benzo[c]phenanthridine alkaloids

SLUNSKÁ, Zdenka, Iva SLANINOVÁ and Eva TÁBORSKÁ. Cytotoxic and colour properties of quaternary benzo[c]phenanthridine alkaloids. Online. In Synthetic and natural compounds in cancer therapy and prevention. 2007. ISBN 978-80-969663-2-5. [citováno 2024-04-24]

Basic information

• Original name: Cytotoxic and colour properties of quaternary benzo[c]phenanthridine alkaloids
• Name in Czech: Cytotoxicita a barevné vlastnosti kvarterních benzo[c]phenanthridinových alkaloidů
• Authors: SLUNSKÁ, Zdenka, Iva SLANINOVÁ and Eva TÁBORSKÁ
• Edition: Synthetic and natural compounds in cancer therapy and prevention, 2007.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• ISBN: 978-80-969663-2-5
• Keywords in English: benzo[c]phenanthridine alkaloids
• Tags: Benzo[c]phenanthridine Alkaloids

Abstract

Our results show that IC50 values (0,03-3,7 microg/ml) after 72 hours treatment of alkaloids are comparable with IC50 of routinely used cytostatics. Cytotoxicity of individual tested alkaloids descended in this order: MA ; SL ; CHL ; SR ; CHR ; SA ;CHE. HL-60 and A-2780 were the most sensitive cell lines while HeLa cells were the most resistant. All tested alkaloids appeared to be inductors of apoptosis at the concentration 1 microg/ml. In addition, we have observed interesting fluorescent characteristics of some QBA upon intercalating into DNA of living cells. We have proved that SA, MA, CHR and partly SR, can be used as supravital fluorescent DNA probes both in fluorescence microscopy and flow cytometry.

Abstract (in Czech)

Benzo[c]phenanthridinové alkaloidy vykazují výraznou cytotoxicitu na nádovových i nenádorových buněčných liniích a zároveň mají významné fluorescenční vlastnosti.

Links

• GA525/04/0017, research and development project: Name: Rostlinné zdroje benzofenanthridinových alkaloidů a účinek těchto alkaloidů na normální a nádorové buňky

Investor: Czech Science Foundation, Plant sources of benzophenanthridine alkaloids and effects of these alkaloid on normal and cancer cells

• GD301/03/H005, research and development project: Name: Molekulární a buněčná biologie v biomedicíně

Investor: Czech Science Foundation, Molecular and cell biology in biomedicine

• LC06077, research and development project: Name: Centrum chemické genetiky

Investor: Ministry of Education, Youth and Sports of the CR

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations