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@inproceedings{720552, author = {Klepárník, Martin and Tomandl, Josef}, address = {Olomouc}, booktitle = {Book of Abstracts}, keywords = {assymetric dimethylarginine; HPLC}, language = {eng}, location = {Olomouc}, isbn = {978-80-244-1705-9}, pages = {98-98}, publisher = {Palacký University Olomouc}, title = {Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection}, url = {http://analytika.upol.cz/chiranal/}, year = {2007} }
TY - JOUR ID - 720552 AU - Klepárník, Martin - Tomandl, Josef PY - 2007 TI - Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection PB - Palacký University Olomouc CY - Olomouc SN - 9788024417059 KW - assymetric dimethylarginine KW - HPLC UR - http://analytika.upol.cz/chiranal/ N2 - Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection. ER -
KLEPÁRNÍK, Martin a Josef TOMANDL. Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection. In \textit{Book of Abstracts}. Olomouc: Palacký University Olomouc, 2007, s.~98-98. ISBN~978-80-244-1705-9.
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