Detailed Information on Publication Record
2007
Cytochrome P450 2C9 Activity Study by Micellar Electrokinetic Capillary Chromatography (Lecture)
KONEČNÝ, Jiří and Zdeněk GLATZBasic information
Original name
Cytochrome P450 2C9 Activity Study by Micellar Electrokinetic Capillary Chromatography (Lecture)
Name in Czech
Stanovení aktivity cytochromu P450 2C9 pomocí MEKC (Přednáška)
Authors
KONEČNÝ, Jiří and Zdeněk GLATZ
Edition
Olomouc, Book of Abstracts, p. 98-98, 1 pp. 2007
Publisher
Palacký University Olomouc
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10406 Analytical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Science
ISBN
978-80-244-1705-9
Keywords in English
assymetric dimethylarginine; HPLC
Tags
International impact
Změněno: 19/5/2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.
V originále
Cytochromes P45O are involved in a wide variety of metabolic and biosynthetic processes encompassing drug metabolism. Cytochrome P450 2C9 (CYP2C9) is one of the most important isoform in human liver involved in the metabolism of a large number of therapeutic agents. Predominantly high performance liquid chromatography (HPLC) with UV and/or fluorescence detection is chosen for these studies. By reason of sample saving and minor time demands, the possibility of automation, CE analysis is suitable for studies comprising work with enzymes, substrates as well as inhibitors. The applicability of capillary electrophoresis (CE) for the determination of enzymatic activity of CYP2C9 with diclofenac as a probe substrate is demonstrated. Micellar electrokinetic capillary chromatography (MEKC) with SDS as a pseudostationary phase was used for this purpose. The kinetic study on the given enzymatic reaction was also performed since basic characterization of drug biotransformation generally begins with the enzyme kinetic analysis of metabolite formation. As a result Michaelis constant and maximum reaction velocity were evaluated, the values 3.44 uM respectively 19.78 nmol.min-1.nmol-1 were in agreement with the literature data. Moreover, the proposed method is suitable for assessing of substrate inhibition study using sulfaphenazole as an inhibitor of CYP2C9 activity. As a result the IC50 value for sulfaphenazole was determinated 0.29 uM which is in consistence with those reported in literature.
In Czech
Byla vypracována metoda stanovení aktivity cytochromu P450 2C9 pomocí MEKC.
Links
| GA203/06/0047, research and development project |
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| LC06023, research and development project |
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| MSM0021622413, plan (intention) |
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