HYRŠL, Pavel, Ender BÜYÜKGÜZEL and Kemal BÜYÜKGÜZEL. Boric acid-induced effects on protein profiles of Galleria mellonella hemolymph and fat body. Acta Biologica Hungarica. Budapest: Akademiai Kiado, 2008, vol. 59, No 3, p. 281-288. ISSN 0236-5383.
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Basic information
Original name Boric acid-induced effects on protein profiles of Galleria mellonella hemolymph and fat body
Name in Czech Efekty indukované kyselinou boritou na proteinové profily hemolymfy a tukového tělesa G. mellonella
Authors HYRŠL, Pavel (203 Czech Republic, guarantor), Ender BÜYÜKGÜZEL (792 Turkey) and Kemal BÜYÜKGÜZEL (792 Turkey).
Edition Acta Biologica Hungarica, Budapest, Akademiai Kiado, 2008, 0236-5383.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30105 Physiology
Country of publisher Hungary
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.619
RIV identification code RIV/00216224:14310/08:00032220
Organization unit Faculty of Science
UT WoS 000259786300002
Keywords in English Galleria mellonella; boric acid; protein profiles; hemolymph; fat body
Tags boric acid, fat body, Galleria mellonella, hemolymph, protein profiles
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Pavel Hyršl, Ph.D., učo 9982. Changed: 11/4/2010 16:42.
Abstract
The dietary effects of boric acid (BA) on the protein profiles of greater wax moth, Galleria mellonella (L.), were investigated in hemolymph and fat body of final instar (VIIth) and pupae. The insects were reared from first-instar larvae on an artificial diets containing 156, 620, 1250 or 2500 ppm of BA. We detected many undetermined protein fractions (6.5 - 260 kDa) in addition to well-defined protein fractions such as lipophorins and storage proteins in the tissues by using sodium dodecyl-sulphate polyacrylamide gradient gel electrophoresis. A marked quantitative change in the 45 kDa protein fraction of the hemolymph was observed in the VIIth instar larvae reared on 2500 ppm dietary BA.
Abstract (in Czech)
Byl zkoumán efekt kyseliny borité (BA) na proteinové profily hemolymfy a tukového tělesa larev sedmého instaru a kukel zavíječe voskového Galleria mellonella (L.). Larvy byly chovány na umělé potravě obsahující 156, 620, 1250 nebo 2500 ppm BA. Pomocí SDS-PAGGE jsme detekovali blíže neurčené proteinové frakce v rozmezí 6.5 - 260 kDa a také lipophoriny a zásobní proteiny hemolymfy. U larev sedmého instaru chovaných na dietě s 2500 ppm BA byla zjištěna kvantitativní změna proteinové frakce 45 kDa.
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