Correlation of selected microRNAs with histopathologic features
of colorectal cancer

D 2007

Correlation of selected microRNAs with histopathologic features of colorectal cancer

SLABÝ, Ondřej, Marek SVOBODA, Pavel FABIAN, Miroslav SVOBODA, Ingrid GARAJOVÁ et. al.

Basic information

Original name

Correlation of selected microRNAs with histopathologic features of colorectal cancer

Name in Czech

Korelace vybraných mikroRNA s histopatologickými parametry u karcinomu tlustého střeva a konečníku.

Authors

SLABÝ, Ondřej, Marek SVOBODA, Pavel FABIAN, Miroslav SVOBODA, Ingrid GARAJOVÁ, Milana ŠACHLOVÁ, Tamara ŠMERDOVÁ, Knoflíčková DANA and Rostislav VYZULA

Edition

Brno, ANALYTICAL CYTOMETRY IV. BOOK OF ABSTRACTS. p. 131-131, 1 pp. 2007

Publisher

Czech Society for Analytical Cytology

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

ISBN

978-80-239-9591-6

Keywords in English

colorectal cancer; patogenesis; microRNA

Abstract

ORIG CZ

V originále

PURPOSE: MicroRNAs (miRNAs) are endogenously expressed regulatory non-coding RNAs, 18 to 25 nucleotides in length, that repress protein translation through binding to target mRNAs. miRNAs have been implicated in many cellular processes including cell proliferation, differentiation and apoptosis. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the potential involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). PATIENTS AND METHODS: In our study, we examined by real-time PCR expression levels of miR21, miR31, miR143, miR145 and let7a1 in bioptic samples of 29 colorectal cancer patients including 3 cases of IUCC Stage I, 11 of Stage II, 6 of Stage III, 9 of Stage IV. For 6 cases of CRC samples also adjacent non-tumor tissue was analyzed. MiRNAs expression levels were correlated with tumor stage, grade, size, anatomical localization and serum CEA levels. For data normalization we tried different approaches (18S rRNA, GAPDH, let7a1), but finally variability of let7a1 expression was shown to be the lowest. P values were calculated using Mann-Whitney U test. RESULTS: Expression levels of all analyzed miRNAs significantly differ in tumor and normal mucosa, miR21 (p=0,0001) and miR31 (p=0,0006) were up-regulated and miR143 (p=0,013) and miR145 (p=0,018) were downregulated in tumors. MiR21 was also correlated with CRC stage. Although the highest levels of miR143 and miR-145 were in normal mucosa, we identified positive correlation of tumor stage and their expression suggesting altered tumor suppressor function of these miRNAs in early events of colorectal carcinogenesis. Distal CRC showed significant upregulation of miR31 (p=0,024) expression. Higher serum levels of CEA were associated with down-regulation of miR145 (p=0,05). We have not associated any of studied miRNAs to tumor grade and tumor size. Tumors with down-regulated miR143 and miR145 were bigger and more frequent (not significantly) in proximal colon. CONCLUSIONS: Our results suggest possible roles of miR21, miR31, miR143 and miR145 in colorectal cancer pathogenesis and different histopathologic phenotypes. This work was supported by IGA MZ CR NR/9076-4

In Czech

Publikace je pouze v anglickém jazyce.

Links

NR9076, research and development project

Name: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku

Citovat

SLABÝ, Ondřej, Marek SVOBODA, Pavel FABIAN, Miroslav SVOBODA, Ingrid GARAJOVÁ, Milana ŠACHLOVÁ, Tamara ŠMERDOVÁ, Knoflíčková DANA and Rostislav VYZULA. Correlation of selected microRNAs with histopathologic features of colorectal cancer. In ANALYTICAL CYTOMETRY IV. BOOK OF ABSTRACTS. Brno: Czech Society for Analytical Cytology, 2007, p. 131-131. ISBN 978-80-239-9591-6.

@inproceedings{720708,
   author = {Slabý, Ondřej and Svoboda, Marek and Fabian, Pavel and Svoboda, Miroslav and Garajová, Ingrid and Šachlová, Milana and Šmerdová, Tamara and Dana, Knoflíčková and Vyzula, Rostislav},
   address = {Brno},
   booktitle = {ANALYTICAL CYTOMETRY IV. BOOK OF ABSTRACTS.},
   keywords = {colorectal cancer; patogenesis; microRNA},
   language = {eng},
   location = {Brno},
   isbn = {978-80-239-9591-6},
   pages = {131-131},
   publisher = {Czech Society for Analytical Cytology},
   title = {Correlation of selected microRNAs with histopathologic features of colorectal cancer},
   year = {2007}
}

TY  - JOUR
ID  - 720708
AU  - Slabý, Ondřej - Svoboda, Marek - Fabian, Pavel - Svoboda, Miroslav - Garajová, Ingrid - Šachlová, Milana - Šmerdová, Tamara - Dana, Knoflíčková - Vyzula, Rostislav
PY  - 2007
TI  - Correlation of selected microRNAs with histopathologic features of colorectal cancer
PB  - Czech Society for Analytical Cytology
CY  - Brno
SN  - 9788023995916
KW  - colorectal cancer
KW  - patogenesis
KW  - microRNA
N2  - PURPOSE: MicroRNAs (miRNAs) are endogenously expressed regulatory non-coding RNAs, 18 to 25 nucleotides in length, that repress protein translation through binding to target mRNAs. miRNAs have been implicated in many cellular processes including cell proliferation, differentiation and apoptosis. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the potential involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). PATIENTS AND METHODS: In our study, we examined by real-time PCR expression levels of miR21, miR31, miR143, miR145 and let7a1 in bioptic samples of 29 colorectal cancer patients including 3 cases of IUCC Stage I, 11 of Stage II, 6 of Stage III, 9 of Stage IV. For 6 cases of CRC samples also adjacent non-tumor tissue was analyzed. MiRNAs expression levels were correlated with tumor stage, grade, size, anatomical localization and serum CEA levels. For data normalization we tried different approaches (18S rRNA, GAPDH, let7a1), but finally variability of let7a1 expression was shown to be the lowest. P values were calculated using Mann-Whitney U test. RESULTS: Expression levels of all analyzed miRNAs significantly differ in tumor and normal mucosa, miR21 (p=0,0001) and miR31 (p=0,0006) were up-regulated and miR143 (p=0,013) and miR145 (p=0,018) were downregulated in tumors. MiR21 was also correlated with CRC stage. Although the highest levels of miR143 and miR-145 were in normal mucosa, we identified positive correlation of tumor stage and their expression suggesting altered tumor suppressor function of these miRNAs in early events of colorectal carcinogenesis. Distal CRC showed significant upregulation of miR31 (p=0,024) expression. Higher serum levels of CEA were associated with down-regulation of miR145 (p=0,05). We have not associated any of studied miRNAs to tumor grade and tumor size. Tumors with down-regulated miR143 and miR145 were bigger and more frequent (not significantly) in proximal colon. CONCLUSIONS: Our results suggest possible roles of miR21, miR31, miR143 and miR145 in colorectal cancer pathogenesis and different histopathologic phenotypes. This work was supported by IGA MZ CR NR/9076-4
ER  -

SLABÝ, Ondřej, Marek SVOBODA, Pavel FABIAN, Miroslav SVOBODA, Ingrid GARAJOVÁ, Milana ŠACHLOVÁ, Tamara ŠMERDOVÁ, Knoflíčková DANA and Rostislav VYZULA. Correlation of selected microRNAs with histopathologic features of colorectal cancer. In \textit{ANALYTICAL CYTOMETRY IV. BOOK OF ABSTRACTS.}. Brno: Czech Society for Analytical Cytology, 2007, p.~131-131. ISBN~978-80-239-9591-6.

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