Is GC-content Correlated with Genome Size in Plants

BUREŠ, Petr, Petr ŠMARDA, Ivana HRALOVÁ, Klára HELÁNOVÁ, Sara FUENTES-SORIANO and Jana PROCHÁZKOVÁ. Is GC-content Correlated with Genome Size in Plants. In Plant Genome Horizons - Vistas & Visions. 2007.

Basic information

• Original name: Is GC-content Correlated with Genome Size in Plants
• Name in Czech: Koreluje obsah guaninu a cytosinu s velikostí genomu u rostlin?
• Authors: BUREŠ, Petr (203 Czech Republic, guarantor, belonging to the institution), Petr ŠMARDA (203 Czech Republic, belonging to the institution), Ivana HRALOVÁ (203 Czech Republic), Klára HELÁNOVÁ (203 Czech Republic, belonging to the institution), Sara FUENTES-SORIANO (484 Mexico) and Jana PROCHÁZKOVÁ (203 Czech Republic).
• Edition: Plant Genome Horizons - Vistas & Visions, 2007.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/07:00022337
• Organization unit: Faculty of Science
• Keywords in English: GC-content; Angiosperms; base composition; c-value; Apiaceae; Brassicaceae; Carex; Eleocharis; Festuca; Epilobium; Festuca
• Tags: angiosperms, Apiaceae, base composition, Brassicaceae, C-value, Carex, Eleocharis, Epilobium, Festuca, GC-content
• Tags: International impact

Abstract

GC base pair content is an important character in the description of new taxa of prokaryotes, but its role in plant systematics and evolution is still poorly understood. Although GC content is positively correlated with genome size in bacteria and vertebrates (1) there is debate about how those two factors are related in plants. Barow and Meister (2) estimated base composition and genome size in 54 taxa of angiosperms and gymnosperms, and their findings did not support Vinogradov (1) proposition stating that there is a positive correlation between GC content and genome size. More recently, Barow and Meister (3) confirmed that base composition and genome size were not correlated using an expanded sampling of 215 plant species measured by numerous authors over the last 14 years. Using flow cytometry methods (FCM) with PI and DAPI, we estimated base composition in closely related species of Apiaceae (16 spp.), Brassicaceae (30 spp.), Cyperaceae (135 spp.), Onagraceae (15 spp.), Poaceae (132 spp.) and Salicaceae (20 spp.), and found a significant positive correlation between GC content and either genome size or monoploid genome size. Sequence data of 11 Oryza species (4) independently supported our FCM results and suggested that GC and genome size correlation is explained by genomic repeats and interspecific genome size variation found within closely related taxa. 1. Vinogradov AE (1994): Cytometry 16: 34-40. 2. Barow M & Meister A (2002): Cytometry 47: 1-7. 3. Meister A & Barow M (2007): Analysis of Base Composition by Flow Cytometry. In: Doležel J et al. (eds.), Flow Cytometry with Plant Cells. Wiley, Weinheim, pp. 177-215. 4. Ammiraju JSS et al. (2006): Genome Research 16: 140-147.

Abstract (in Czech)

GC base pair content is an important character in the description of new taxa of prokaryotes, but its role in plant systematics and evolution is still poorly understood. Although GC content is positively correlated with genome size in bacteria and vertebrates (1) there is debate about how those two factors are related in plants. Barow and Meister (2) estimated base composition and genome size in 54 taxa of angiosperms and gymnosperms, and their findings did not support Vinogradov (1) proposition stating that there is a positive correlation between GC content and genome size. More recently, Barow and Meister (3) confirmed that base composition and genome size were not correlated using an expanded sampling of 215 plant species measured by numerous authors over the last 14 years. Using flow cytometry methods (FCM) with PI and DAPI, we estimated base composition in closely related species of Apiaceae (16 spp.), Brassicaceae (30 spp.), Cyperaceae (135 spp.), Onagraceae (15 spp.), Poaceae (132 spp.) and Salicaceae (20 spp.), and found a significant positive correlation between GC content and either genome size or monoploid genome size. Sequence data of 11 Oryza species (4) independently supported our FCM results and suggested that GC and genome size correlation is explained by genomic repeats and interspecific genome size variation found within closely related taxa. 1. Vinogradov AE (1994): Cytometry 16: 34-40. 2. Barow M & Meister A (2002): Cytometry 47: 1-7. 3. Meister A & Barow M (2007): Analysis of Base Composition by Flow Cytometry. In: Doležel J et al. (eds.), Flow Cytometry with Plant Cells. Wiley, Weinheim, pp. 177-215. 4. Ammiraju JSS et al. (2006): Genome Research 16: 140-147.

Links

• LC06073, research and development project: Name: Centrum pro výzkum biodiverzity

Investor: Ministry of Education, Youth and Sports of the CR, Biodiversity Research Center

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time