D 2007

Multidetection Platform for Microcolumn Separation of Proteins and Peptides

PREISLER, Jan, Pavel KRÁSENSKÝ, Patrik VRÁBEL, Ondřej PEŠ, Tomáš VACULOVIČ et. al.

Basic information

Original name

Multidetection Platform for Microcolumn Separation of Proteins and Peptides

Name in Czech

Multidetekční platforma pro mikrokolonové separace proteinů a peptidů

Edition

1. vyd. Štrbské Pleso, High Tatras, Slovakia, Book of Abstracts and Abbreviated Papers 13th ISSS & 13th AACI, p. 38-38, 2007

Publisher

Slovak University of Technology, Bratislava, Slovakia

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

ISBN

978-80-227-2698-6

Keywords in English

microcolumn; separation; mass spectrometry; MALDI; CE; HPLC; fluorescence; LIF; inductively-coupled plasma

Tags

International impact
Změněno: 9/4/2013 14:15, prof. Mgr. Jan Preisler, Ph.D.

Abstract

V originále

The lecture describes an instrumentation platform based on a universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides can be analyzed in off-line mode using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification.

In Czech

Přednáška se týkala instrumentální platformy pro off-line spojení mikrokolnových separací s detekcí MS, MS/MS, LIF aj.

Links

LC06034, research and development project
Name: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations