Detailed Information on Publication Record
2007
A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples
KAŇUCH, Peter, Petra HÁJKOVÁ, Zdeněk ŘEHÁK and Josef BRYJABasic information
Original name
A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples
Name in Czech
Rychlý test na druhovou identifikaci dvou kryptických netopýrů Pipistrellus pipistrellus a P. pygmaeus na základě PCR metody a jeho využití na muzejních vzorcích a vzorcích trusu
Authors
KAŇUCH, Peter (703 Slovakia), Petra HÁJKOVÁ (703 Slovakia), Zdeněk ŘEHÁK (203 Czech Republic, guarantor) and Josef BRYJA (203 Czech Republic)
Edition
Acta Chiropterologica, Warszawa, Museum and Institute of Zoology PAS, 2007, 1508-1109
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Poland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.857
RIV identification code
RIV/00216224:14310/07:00020398
Organization unit
Faculty of Science
UT WoS
000249022100022
Keywords in English
Chiroptera; cytochrome b; mtDNA; sibling species; non-invasive sampling
Tags
International impact, Reviewed
Změněno: 28/1/2008 13:56, doc. RNDr. Zdeněk Řehák, Ph.D.
V originále
The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology.
In Czech
The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology.
Links
| GA206/06/0954, research and development project |
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| MSM0021622416, plan (intention) |
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