GROCHOVÁ, Diana, Jana VAŇKOVÁ, Jiří DAMBORSKÝ, Barbora RAVČUKOVÁ, Jan ŠMARDA, Bořivoj VOJTĚŠEK and Jana ŠMARDOVÁ. Analysis of transactivation capability and conformation of p53 temperature-dependent mutants and their reactivation by amifostine in yeast. Oncogene. USA: Nature Publishing Group, 2008, vol. 27, No 9, p. 1243-1252. ISSN 0950-9232. |
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@article{721376, author = {Grochová, Diana and Vaňková, Jana and Damborský, Jiří and Ravčuková, Barbora and Šmarda, Jan and Vojtěšek, Bořivoj and Šmardová, Jana}, article_location = {USA}, article_number = {9}, keywords = {p53; FASAY; transactivation; conformation; temperature-dependent mutants; discriminating mutants}, language = {eng}, issn = {0950-9232}, journal = {Oncogene}, title = {Analysis of transactivation capability and conformation of p53 temperature-dependent mutants and their reactivation by amifostine in yeast}, volume = {27}, year = {2008} }
TY - JOUR ID - 721376 AU - Grochová, Diana - Vaňková, Jana - Damborský, Jiří - Ravčuková, Barbora - Šmarda, Jan - Vojtěšek, Bořivoj - Šmardová, Jana PY - 2008 TI - Analysis of transactivation capability and conformation of p53 temperature-dependent mutants and their reactivation by amifostine in yeast JF - Oncogene VL - 27 IS - 9 SP - 1243-1252 EP - 1243-1252 PB - Nature Publishing Group SN - 09509232 KW - p53 KW - FASAY KW - transactivation KW - conformation KW - temperature-dependent mutants KW - discriminating mutants N2 - The p53 gene is often mutated during cancer development. Frequency and functional consequences of these mutations vary in different tumor types. We analyzed conformation and temperature dependency of 23 partially inactivating temperature-dependent (td) p53 mutants derived from various human tumors in yeast. We found considerable differences in transactivation capabilities and discriminative character of various p53 mutants. No correlations in transactivation rates and conformations of the td p53 proteins were detected. Amifostine-induced p53 reactivation occurred only in 13 of 23 td mutants, and this effect was temperature-dependent and responsive element-specific. The most of the p53 mutations (10/13) reactivated by amifostine were located in the part of the p53 gene coding for hydrophobic beta-sandwich structure of the DNA-binding domain. ER -
GROCHOVÁ, Diana, Jana VAŇKOVÁ, Jiří DAMBORSKÝ, Barbora RAVČUKOVÁ, Jan ŠMARDA, Bořivoj VOJTĚŠEK and Jana ŠMARDOVÁ. Analysis of transactivation capability and conformation of p53 temperature-dependent mutants and their reactivation by amifostine in yeast. \textit{Oncogene}. USA: Nature Publishing Group, 2008, vol.~27, No~9, p.~1243-1252. ISSN~0950-9232.
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