Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry

PETRLOVÁ, Jitka, Michal MASAŘÍK, David POTĚŠIL, Vojtěch ADAM, Libuše TRNKOVÁ and René KIZEK. Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry. Electroanalysis. Germany, Weinheim: Wiley-VCH Verlag GmbH & Co., 2007, vol. 19, No 11, p. 1177-1182. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.200603837.

Basic information

• Original name: Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry
• Name in Czech: Detekce streptavidinu v zeptomolových množstvích s použitím uhlíkové pastové elektrody a square wave voltametrie
• Authors: PETRLOVÁ, Jitka (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Vojtěch ADAM (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution) and René KIZEK (203 Czech Republic).
• Edition: Electroanalysis, Germany, Weinheim, Wiley-VCH Verlag GmbH & Co. 2007, 1040-0397.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10405 Electrochemistry
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.949
• RIV identification code: RIV/00216224:14310/07:00050796
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/elan.200603837
• UT WoS: 000247105000007
• Keywords in English: streptavidin ; avidin ; modified electrode ; electrochemistry ; protein ; carbon paste electrode
• Tags: AKR, Avidin, carbon paste electrode, electrochemistry, modified electrode, PROTEIN, rivok, streptavidin
• Tags: International impact, Reviewed

Abstract

We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination - analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS).

Abstract (in Czech)

V této studii jsme porovnávali citlivost detekce avidinu a streptavidinu pro avidin-biotinovou technologii. Z našich předchozích výsledků je zřejmé, že streptavidin ve srovnání s avidinem dává při elektrochemickém stanovení 1000x vyšší odezvu za stejných podmínek i stejné koncentraci. Zvolili jsme dva přístupy - jednak stanovení streptavidinu v elektrochemické nádobce a jednak stanovení přímo na povrchu pracovní elektrody (přenosová technika). Limity detekce byly v případě elektrochemické nádobky 0.3 aM a v případě přenosové techniky 30 aM.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)