The determination of avidin in genetically modified maize

J 2007

The determination of avidin in genetically modified maize

PETRLOVÁ, Jitka, Soňa KŘÍŽKOVÁ, Veronika ŠUPÁLKOVÁ, Michal MASAŘÍK, Vojtěch ADAM et. al.

Basic information

Original name

The determination of avidin in genetically modified maize

Name in Czech

Stanovení avidinu v geneticky modifikované kukuřici

Authors

PETRLOVÁ, Jitka (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic, belonging to the institution), Veronika ŠUPÁLKOVÁ (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Vojtěch ADAM (203 Czech Republic, belonging to the institution), Ladislav HAVEL (203 Czech Republic), Karl KRAMER (840 United States of America) and René KIZEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant, Soil and Environment, Praha, Czech Academy of Agricultural Sciences, 2007, 1214-1178

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.170 in 2004

RIV identification code

RIV/00216224:14310/07:00050797

Organization unit

Faculty of Science

UT WoS

000254859700003

Keywords in English

avidin; maize; square-wave voltammetry; carbon paste electrode; GMO

Abstract

ORIG CZ

V originále

Quality assurance is a major issue in the food industry. The authenticity of food ingredients and their traceability are required by consumers and authorities. Plant species such as barley (Hordeum vulgare), rice (Oryza sativa), sunflower (Helianthus annus), wheat (Triticum aestivum) and maize (Zea mays) are very common objects of interest of genetic modification (GMO); therefore the development of specific assays for their specific detection and quantification of GMO are needed. Furthermore, the production and trade of genetically modified lines from an increasing number of plant species brings about the need for control within research, environmental risk assessment, labelinglegal,and consumers information purposes. Electrochemical sensors and biosensors based on modification of working electrode could be suitable tools for these purposes. Here, we report using of an avidin-modified carbon paste electrode for rapid and sensitive determination of avidin in plant extract solution and in a transgenic maize extract. The process could be used to determine avidin concentrations up to 3pM in solution and 170nM in a maize seed extract. Moreover, we applied the method to analyze different maize flours.

In Czech

Cílem této práce je analýza avidinu z transgenní kukuřice, do které byl vnesen gen pro avidin a příprava avidinem modifikované pastové uhlíkové elektrody.

Citovat

PETRLOVÁ, Jitka, Soňa KŘÍŽKOVÁ, Veronika ŠUPÁLKOVÁ, Michal MASAŘÍK, Vojtěch ADAM, Ladislav HAVEL, Karl KRAMER and René KIZEK. The determination of avidin in genetically modified maize. Plant, Soil and Environment. Praha: Czech Academy of Agricultural Sciences, 2007, vol. 53, No 8, p. 345-349. ISSN 1214-1178.

@article{721772,
   author = {Petrlová, Jitka and Křížková, Soňa and Šupálková, Veronika and Masařík, Michal and Adam, Vojtěch and Havel, Ladislav and Kramer, Karl and Kizek, René},
   article_location = {Praha},
   article_number = {8},
   keywords = {avidin; maize; square-wave voltammetry; carbon paste electrode; GMO},
   language = {eng},
   issn = {1214-1178},
   journal = {Plant, Soil and Environment},
   title = {The determination of avidin in genetically modified maize},
   volume = {53},
   year = {2007}
}

TY  - JOUR
ID  - 721772
AU  - Petrlová, Jitka - Křížková, Soňa - Šupálková, Veronika - Masařík, Michal - Adam, Vojtěch - Havel, Ladislav - Kramer, Karl - Kizek, René
PY  - 2007
TI  - The determination of avidin in genetically modified maize
JF  - Plant, Soil and Environment
VL  - 53
IS  - 8
SP  - 345-349
EP  - 345-349
PB  - Czech Academy of Agricultural Sciences
SN  - 12141178
KW  - avidin
KW  - maize
KW  - square-wave voltammetry
KW  - carbon paste electrode
KW  - GMO
N2  - Quality assurance is a major issue in the food industry. The authenticity of food ingredients and their traceability are required by consumers and authorities. Plant species such as barley (Hordeum vulgare), rice (Oryza sativa), sunflower (Helianthus annus), wheat (Triticum aestivum) and maize (Zea mays) are very common objects of interest of genetic modification (GMO); therefore the development of specific assays for their specific detection and quantification of GMO are needed. Furthermore, the production and trade of genetically modified lines from an increasing number of plant species brings about the need for control within research, environmental risk assessment, labelinglegal,and consumers information purposes. Electrochemical sensors and biosensors based on modification of working electrode could be suitable tools for these purposes. Here, we report using of an avidin-modified carbon paste electrode for rapid and sensitive determination of avidin in plant extract solution and in a transgenic maize extract. The process could be used to determine avidin concentrations up to 3pM in solution and 170nM in a maize seed extract. Moreover, we applied the method to analyze different maize flours.
ER  -

PETRLOVÁ, Jitka, Soňa KŘÍŽKOVÁ, Veronika ŠUPÁLKOVÁ, Michal MASAŘÍK, Vojtěch ADAM, Ladislav HAVEL, Karl KRAMER and René KIZEK. The determination of avidin in genetically modified maize. \textit{Plant, Soil and Environment}. Praha: Czech Academy of Agricultural Sciences, 2007, vol.~53, No~8, p.~345-349. ISSN~1214-1178.

Detailed Information on Publication Record