PETRLOVÁ, Jitka, Soňa KŘÍŽKOVÁ, Veronika ŠUPÁLKOVÁ, Michal MASAŘÍK, Vojtěch ADAM, Ladislav HAVEL, Karl KRAMER and René KIZEK. The determination of avidin in genetically modified maize. Plant, Soil and Environment. Praha: Czech Academy of Agricultural Sciences, 2007, vol. 53, No 8, p. 345-349. ISSN 1214-1178.
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Basic information
Original name The determination of avidin in genetically modified maize
Name in Czech Stanovení avidinu v geneticky modifikované kukuřici
Authors PETRLOVÁ, Jitka (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic, belonging to the institution), Veronika ŠUPÁLKOVÁ (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Vojtěch ADAM (203 Czech Republic, belonging to the institution), Ladislav HAVEL (203 Czech Republic), Karl KRAMER (840 United States of America) and René KIZEK (203 Czech Republic, guarantor, belonging to the institution).
Edition Plant, Soil and Environment, Praha, Czech Academy of Agricultural Sciences, 2007, 1214-1178.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.170 in 2004
RIV identification code RIV/00216224:14310/07:00050797
Organization unit Faculty of Science
UT WoS 000254859700003
Keywords in English avidin; maize; square-wave voltammetry; carbon paste electrode; GMO
Tags AKR, Avidin, carbon paste electrode, GMO, maize, rivok, Square-Wave Voltammetry
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 13/4/2012 12:25.
Abstract
Quality assurance is a major issue in the food industry. The authenticity of food ingredients and their traceability are required by consumers and authorities. Plant species such as barley (Hordeum vulgare), rice (Oryza sativa), sunflower (Helianthus annus), wheat (Triticum aestivum) and maize (Zea mays) are very common objects of interest of genetic modification (GMO); therefore the development of specific assays for their specific detection and quantification of GMO are needed. Furthermore, the production and trade of genetically modified lines from an increasing number of plant species brings about the need for control within research, environmental risk assessment, labelinglegal,and consumers information purposes. Electrochemical sensors and biosensors based on modification of working electrode could be suitable tools for these purposes. Here, we report using of an avidin-modified carbon paste electrode for rapid and sensitive determination of avidin in plant extract solution and in a transgenic maize extract. The process could be used to determine avidin concentrations up to 3pM in solution and 170nM in a maize seed extract. Moreover, we applied the method to analyze different maize flours.
Abstract (in Czech)
Cílem této práce je analýza avidinu z transgenní kukuřice, do které byl vnesen gen pro avidin a příprava avidinem modifikované pastové uhlíkové elektrody.
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