13C detected NMR experiments for measuring chemical shifts and
coupling constants in nucleic acid bases

J 2007

13C detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases

FIALA, Radovan and Vladimír SKLENÁŘ

Basic information

Original name

13C detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases

Name in Czech

NMR experimenty s uhlíkovou detekcí pro měření chemických posunů a interakčních konstant v bazích nukleových kyselin

Authors

FIALA, Radovan (203 Czech Republic) and Vladimír SKLENÁŘ (203 Czech Republic, guarantor)

Edition

Journal of Biomolecular NMR, Dordrecht, Springer Netherlands, 2007, 0925-2738

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.433

RIV identification code

RIV/00216224:14310/07:00022568

Organization unit

Faculty of Science

Keywords in English

Chemical shift Direct carbon detection NMR Nucleic acid Quaternary carbon Spin spin coupling

Abstract

ORIG CZ

V originále

The paper presents a set of two dimensional experiments that utilize direct 13C detection to provide proton carbon, carbon carbon and carbon nitrogen correlations in the bases of nucleic acids. The set includes a 13C detected proton carbon correlation experiment for the measurement of 13C 13C couplings, the CaCb experiment for correlating two quaternary carbons, the HCaCb experiment for the 13C 13C correlations in cases where one of the carbons has a proton attached, the HCC TOCSY experiment for correlating a proton with a network of coupled carbons, and a 13C detected 13C 15N correlation experiment for detecting the nitrogen nuclei that cannot be detected via protons. The IPAP procedure is used for extracting the carbon carbon couplings or carbon decoupling in the direct dimension, while the S3E procedure is preferred in the indirect dimension of the carbon nitrogen experiment to obtain the value of the coupling constant. The experiments supply accurate values of 13C and 15N chemical shifts and carbon carbon and carbon nitrogen coupling constants. These values can help to reveal structural features of nucleic acids either directly or via induced changes when the sample is dissolved in oriented media.

In Czech

Článek uvádí nové heteronukleární 2D NMR experimenty pro korelaci všech uhlíků v bázích RNA s využitím příme uhlíkové detekce. Experimenty jsou založeny na jedno, dvou nebo třívazebných interakcích a několikanásobných přenosech magnetizace. Experimenty jsou aplikovatelné i na vzorky v orietovaných fázích.

Links

LC06030, research and development project

Name: Biomolekulární centrum

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

FIALA, Radovan and Vladimír SKLENÁŘ. 13C detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases. Journal of Biomolecular NMR. Dordrecht: Springer Netherlands, 2007, vol. 39, No 4, p. 153-163. ISSN 0925-2738.

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   author = {Fiala, Radovan and Sklenář, Vladimír},
   article_location = {Dordrecht},
   article_number = {4},
   keywords = {Chemical shift Direct carbon detection NMR Nucleic acid Quaternary carbon Spin spin coupling},
   language = {eng},
   issn = {0925-2738},
   journal = {Journal of Biomolecular NMR},
   title = {13C detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases},
   url = {http://springerlink.metapress.com/content/2n4v387l673t185g/?p=739cfdebf3084dcfbe5d88345c966657&pi=6},
   volume = {39},
   year = {2007}
}

TY  - JOUR
ID  - 725000
AU  - Fiala, Radovan - Sklenář, Vladimír
PY  - 2007
TI  - 13C detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases
JF  - Journal of Biomolecular NMR
VL  - 39
IS  - 4
SP  - 153-163
EP  - 153-163
PB  - Springer Netherlands
SN  - 09252738
KW  - Chemical shift Direct carbon detection NMR Nucleic acid Quaternary carbon Spin spin coupling
UR  - http://springerlink.metapress.com/content/2n4v387l673t185g/?p=739cfdebf3084dcfbe5d88345c966657&pi=6
N2  - The paper presents a set of two dimensional experiments that utilize direct 13C detection to provide proton carbon, carbon carbon and carbon nitrogen correlations in the bases of nucleic acids. The set includes a 13C detected proton carbon correlation experiment for the measurement of 13C 13C couplings, the CaCb experiment for correlating two quaternary carbons, the HCaCb experiment for the 13C 13C correlations in cases where one of the carbons has a proton attached, the HCC TOCSY experiment for correlating a proton with a network of coupled carbons, and a 13C detected 13C 15N correlation experiment for detecting the nitrogen nuclei that cannot be detected via protons. The IPAP procedure is used for extracting the carbon carbon couplings or carbon decoupling in the direct dimension, while the S3E procedure is preferred in the indirect dimension of the carbon nitrogen experiment to obtain the value of the coupling constant. The experiments supply accurate values of 13C and 15N chemical shifts and carbon carbon and carbon nitrogen coupling constants. These values can help to reveal structural features of nucleic acids either directly or via induced changes when the sample is dissolved in oriented media.
ER  -

FIALA, Radovan and Vladimír SKLENÁŘ. 13C detected NMR experiments for measuring chemical shifts and coupling constants in nucleic acid bases. \textit{Journal of Biomolecular NMR}. Dordrecht: Springer Netherlands, 2007, vol.~39, No~4, p.~153-163. ISSN~0925-2738.

Detailed Information on Publication Record